Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

David Wright; Zoltan Ferjentsik; Shang Wei Chong; Xuehui Qiu; Yun Jin Jiang; Pascale Malapert; Olivier Pourquié; Nick Van Hateren; Stuart A. Wilson; Claudio Franco; Holger Gerhardt; J. Kim Dale; Miguel Maroto

doi:10.1002/dvdy.22139

Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

David Wright, Zoltan Ferjentsik, Shang Wei Chong, Xuehui Qiu, Yun Jin Jiang, Pascale Malapert, Olivier Pourquié, Nick Van Hateren, Stuart A. Wilson, Claudio Franco, Holger Gerhardt, J. Kim Dale, Miguel Maroto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes.

Original language	English
Pages (from-to)	3043-3055
Number of pages	13
Journal	Developmental Dynamics
Volume	238
Issue number	12
DOIs	https://doi.org/10.1002/dvdy.22139
Publication status	Published - Dec 2009
Externally published	Yes

Keywords

Cyclic gene
Embryo
Notch pathway
Nrarp
PSM
Somitic oscillator

Access to Document

10.1002/dvdy.22139

Cite this

Wright, D., Ferjentsik, Z., Chong, S. W., Qiu, X., Jiang, Y. J., Malapert, P., Pourquié, O., Van Hateren, N., Wilson, S. A., Franco, C., Gerhardt, H., Dale, J. K., & Maroto, M. (2009). Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate. Developmental Dynamics, 238(12), 3043-3055. https://doi.org/10.1002/dvdy.22139

@article{16b59411b03a42a9bd6f6f5ecdc84885,

title = "Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate",

abstract = "Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes.",

keywords = "Cyclic gene, Embryo, Notch pathway, Nrarp, PSM, Somitic oscillator",

author = "David Wright and Zoltan Ferjentsik and Chong, {Shang Wei} and Xuehui Qiu and Jiang, {Yun Jin} and Pascale Malapert and Olivier Pourqui{\'e} and {Van Hateren}, Nick and Wilson, {Stuart A.} and Claudio Franco and Holger Gerhardt and Dale, {J. Kim} and Miguel Maroto",

year = "2009",

month = dec,

doi = "10.1002/dvdy.22139",

language = "English",

volume = "238",

pages = "3043--3055",

journal = "Developmental Dynamics",

issn = "1058-8388",

publisher = "Wiley-Liss Inc.",

number = "12",

}

TY - JOUR

T1 - Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

AU - Wright, David

AU - Ferjentsik, Zoltan

AU - Chong, Shang Wei

AU - Qiu, Xuehui

AU - Jiang, Yun Jin

AU - Malapert, Pascale

AU - Pourquié, Olivier

AU - Van Hateren, Nick

AU - Wilson, Stuart A.

AU - Franco, Claudio

AU - Gerhardt, Holger

AU - Dale, J. Kim

AU - Maroto, Miguel

PY - 2009/12

Y1 - 2009/12

N2 - Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes.

AB - Somites are formed progressively from the presomitic mesoderm (PSM) in a highly regulated process according to a strict periodicity driven by an oscillatory mechanism. The Notch and Wnt pathways are key components in the regulation of this somitic oscillator and data from Xenopus and zebrafish embryos indicate that the Notch-downstream target Nrarp participates in the regulation of both activities. We have analyzed Nrarp/nrarp-a expression in the PSM of chick, mouse and zebrafish embryos, and we show that it cycles in synchrony with other Notch regulated cyclic genes. In the mouse its transcription is both Wnt- and Notch-dependent, whereas in the chick and fish embryo it is simply Notch-dependent. Despite oscillating mRNA levels, Nrarp protein does not oscillate in the PSM. Finally, neither gain nor loss of Nrarp function interferes with the normal expression of Notch-related cyclic genes.

KW - Cyclic gene

KW - Embryo

KW - Notch pathway

KW - Nrarp

KW - PSM

KW - Somitic oscillator

UR - http://www.scopus.com/inward/record.url?scp=70949107839&partnerID=8YFLogxK

U2 - 10.1002/dvdy.22139

DO - 10.1002/dvdy.22139

M3 - Article

C2 - 19882724

SN - 1058-8388

VL - 238

SP - 3043

EP - 3055

JO - Developmental Dynamics

JF - Developmental Dynamics

IS - 12

ER -

Cyclic Nrarp mRNA expression is regulated by the somitic oscillator but Nrarp protein levels do not oscillate

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this