Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes

João C. Fernandes; Margarida Borges; Henrique Nascimento; Elsa Bronze-da-Rocha; Oscar S. Ramos; Manuela E. Pintado; F. Xavier Malcata; Alice Santos-Silva

doi:10.1016/j.ijbiomac.2011.05.032

Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes

João C. Fernandes^*, Margarida Borges, Henrique Nascimento, Elsa Bronze-da-Rocha, Oscar S. Ramos, Manuela E. Pintado, F. Xavier Malcata, Alice Santos-Silva

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07. mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10. mg/mL) and/or apoptosis (<0.10. mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.

Original language	English
Pages (from-to)	433-438
Number of pages	6
Journal	International Journal of Biological Macromolecules
Volume	49
Issue number	3
DOIs	https://doi.org/10.1016/j.ijbiomac.2011.05.032
Publication status	Published - 1 Oct 2011

Keywords

Chitooligosaccharides
Comet assay
Flow cytometry
Lymphocytes
Micronucleus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ijbiomac.2011.05.032

http://hdl.handle.net/10400.14/7952

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title = "Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes",

abstract = "Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07. mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10. mg/mL) and/or apoptosis (<0.10. mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.",

keywords = "Chitooligosaccharides, Comet assay, Flow cytometry, Lymphocytes, Micronucleus",

author = "Fernandes, {Jo{\~a}o C.} and Margarida Borges and Henrique Nascimento and Elsa Bronze-da-Rocha and Ramos, {Oscar S.} and Pintado, {Manuela E.} and Malcata, {F. Xavier} and Alice Santos-Silva",

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TY - JOUR

T1 - Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes

AU - Fernandes, João C.

AU - Borges, Margarida

AU - Nascimento, Henrique

AU - Bronze-da-Rocha, Elsa

AU - Ramos, Oscar S.

AU - Pintado, Manuela E.

AU - Malcata, F. Xavier

AU - Santos-Silva, Alice

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07. mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10. mg/mL) and/or apoptosis (<0.10. mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.

AB - Two COS mixtures and a low molecular weight chitosan (LMWC) were tested for potential cytotoxicity and genotoxicity upon human lymphocytes. Genotoxicity was evaluated in vitro by cytokinesis-blocked micronucleus and alkaline comet assays, while cytotoxicity was assessed by flow cytometry analysis. Our results suggest that COS do not exhibit any genotoxicity upon human lymphocytes, independently of MW or concentration. However, above 0.07. mg/mL COS induced strong cytotoxic effects. According to the concentration used, such cytotoxicity will induce cell death, essentially by necrosis (>0.10. mg/mL) and/or apoptosis (<0.10. mg/mL). The level of necrosis/apoptosis induced by high COS concentrations, suggests a promising use as apoptosis inducers in specific cancer situations.

KW - Chitooligosaccharides

KW - Comet assay

KW - Flow cytometry

KW - Lymphocytes

KW - Micronucleus

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SN - 0141-8130

VL - 49

SP - 433

EP - 438

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

IS - 3

ER -

Cytotoxicity and genotoxicity of chitooligosaccharides upon lymphocytes

Abstract

Keywords

UN SDGs

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