TY - JOUR
T1 - Cytotoxicity evaluation of Curcuma zedoaria (Christm.) Roscoe fluid extract used in oral hygiene products
AU - Dos Santos Fernandes, Joao Paulo
AU - Mello-Moura, Anna Carolina Volpi
AU - Marques, Marcia Martins
AU - Nicoletti, Maria Aparecida
N1 - Funding Information:
The authors wish to thank FAPESP (Fundação de Amparo à Pesquisa do Estado de São Paulo) for the scholarship to J.P.S. Fernandes.
PY - 2012/12
Y1 - 2012/12
N2 - Objective: This in vitro study evaluated the cytotoxic effects of the Curcuma zedoaria (Christm.) Roscoe (popular name: zedoary) fluid extract, as used in preparations for oral hygiene, mostly for anti-septic purposes. Materials and methods: The cell viability and cell growth were assessed by Trypan blue dye exclusion assay using the LMF cell line derived from oral mucosa. Cell viability (short-term assay) was measured 0, 6, 12 and 24 h after contact with the fluid extract. Cell growth (long-term assay) was analyzed in 1, 3, 5 and 7 days. The experimental groups were those testing the fluid extract obtained from the zedoary rhizome and the extractor liquid (ethanol 70° GL) in the concentrations of 0.01-0.0001% v/v. Fresh DMEM were used in the control cultures. Results: Short-term assay - all studied cultures maintained stable cell viability; Longterm assay - there was progressive cell growth in all studied cultures. Conclusion: According to the results, the zedoary fluid extract presents low cytotoxicity and probably can be used in the oral hygiene products.
AB - Objective: This in vitro study evaluated the cytotoxic effects of the Curcuma zedoaria (Christm.) Roscoe (popular name: zedoary) fluid extract, as used in preparations for oral hygiene, mostly for anti-septic purposes. Materials and methods: The cell viability and cell growth were assessed by Trypan blue dye exclusion assay using the LMF cell line derived from oral mucosa. Cell viability (short-term assay) was measured 0, 6, 12 and 24 h after contact with the fluid extract. Cell growth (long-term assay) was analyzed in 1, 3, 5 and 7 days. The experimental groups were those testing the fluid extract obtained from the zedoary rhizome and the extractor liquid (ethanol 70° GL) in the concentrations of 0.01-0.0001% v/v. Fresh DMEM were used in the control cultures. Results: Short-term assay - all studied cultures maintained stable cell viability; Longterm assay - there was progressive cell growth in all studied cultures. Conclusion: According to the results, the zedoary fluid extract presents low cytotoxicity and probably can be used in the oral hygiene products.
KW - Cell culture
KW - Cytotoxicity
KW - Vegetal extract
KW - Zedoary
UR - http://www.scopus.com/inward/record.url?scp=84867292832&partnerID=8YFLogxK
U2 - 10.3109/00016357.2011.645060
DO - 10.3109/00016357.2011.645060
M3 - Article
C2 - 22251086
AN - SCOPUS:84867292832
SN - 0001-6357
VL - 70
SP - 610
EP - 614
JO - Acta Odontologica Scandinavica
JF - Acta Odontologica Scandinavica
IS - 6
ER -