Dataset of the preparation and characterization of an artificial sludge for ecotoxicological purposes

Ana Gavina*, Ana Cristina Freitas, Amadeu Ricardo, Ana Gomes, Catarina Marques, Isabel Lopes, Ruth Pereira

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


This work describes the protocol developed for preparing an artificial digested sludge to be used for the ecotoxicological evaluation and risk assessment of chemical substances that are previewed to attain the soil through sewage sludge applications for fertilization (e.g. pharmaceutical compounds). Such evaluations have been made with standard protocols for ecotoxicological tests with terrestrial species in which the chemical substances are directly spiked to the test soil. This procedure lacks ecological relevance in terms of the role the sludge organic matter plays on the bioavailability and fate of chemical substances. Here we describe the protocol and the composition for obtaining the artificial sludge, prepared with commercial pet food and eggs white to mimic the composition of domestic sewage sludge in terms of proteins, carbon hydrates, fat and fibers content. Further, the conditions ascertained for the anaerobic digestion of the organic mixture are described, and the final properties of the sludge are presented, after repeating the procedure twice.
Original languageEnglish
Article number104385
Number of pages4
JournalData in Brief
Publication statusPublished - Aug 2019


  • Artificial sludge protocol
  • Ecotoxicological tests
  • Risk assessment
  • Soil


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