Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

Maria F. Carvalho; Maria Isabel M. Ferreira; Irina S. Moreira; Paula M. L. Castro; Dick B. Janssen

doi:10.1128/AEM.01162-06

Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

Maria F. Carvalho, Maria Isabel M. Ferreira, Irina S. Moreira, Paula M. L. Castro^*, Dick B. Janssen

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.

Original language	English
Pages (from-to)	7413-7417
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	11
DOIs	https://doi.org/10.1128/AEM.01162-06
Publication status	Published - Nov 2006

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http://hdl.handle.net/10400.14/5518

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title = "Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol",

abstract = "The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.",

author = "Carvalho, {Maria F.} and Ferreira, {Maria Isabel M.} and Moreira, {Irina S.} and Castro, {Paula M. L.} and Janssen, {Dick B.}",

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AU - Carvalho, Maria F.

AU - Ferreira, Maria Isabel M.

AU - Moreira, Irina S.

AU - Castro, Paula M. L.

AU - Janssen, Dick B.

PY - 2006/11

Y1 - 2006/11

N2 - The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.

AB - The aerobic metabolism of fluorobenzene by Rhizobiales sp. strain F11 was investigated. Liquid chromatography-mass spectrometry analysis showed that 4-fluorocatechol and catechol were formed as intermediates during fluorobenzene degradation by cell suspensions. Both these compounds, unlike 3-fluorocatechol, supported growth and oxygen uptake. Cells grown on fluorobenzene contained enzymes for the ortho pathway but not for meta ring cleavage of catechols. The results suggest that fluorobenzene is predominantly degraded via 4-fluorocatechol with subsequent ortho cleavage and also partially via catechol.

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 11

ER -

Degradation of fluorobenzene by Rhizobiales strain F11 via ortho cleavage of 4-fluorocatechol and catechol

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