Designing a multiepitope vaccine against the foodborne pathogenic bacteria Listeria monocytogenes using subtractive immunoinformatics approaches

Tariq Aziz*, Muhammad Naveed, Muhammad Aqib Shabbir, Khizra Jabeen, Ayaz Ali Khan, Ammarah Hasnain, Zhennai Yang, Abdellah Zinedine, João Miguel Rocha*, Thamer H. Albekairi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Background: Listeria monocytogenes, a Gram-positive bacterium, is a prominent foodborne pathogen that causes listeriosis and poses substantial health hazards worldwide. The continuing risk of listeriosis outbreaks underlies the importance of designing an effective prevention strategy and developing a robust immune response by reverse vaccinology approaches. This study aimed to provide a critical approach for developing a potent multiepitope vaccine against this foodborne disease. Methods: A chimeric peptide construct containing 5 B-cell epitopes, 16 major histocompatibility complex I (MHC-I) epitopes, and 18 MHC-II epitopes were used to create a subunit vaccination against L. monocytogenes. The vaccine safety was evaluated by several online methods, and molecular docking was performed using ClusPro to determine the binding affinity. Immune simulation was performed using the C-ImmSimm server to demonstrate the immune response. Results: The results validated the antigenicity, non-allergenicity, and nontoxicity of the chimeric peptide construct, confirming its suitability as a subunit vaccine. Molecular docking showed a good score of 1276.5 and molecular dynamics simulations confirmed the construct’s efficacy, demonstrating its promise as a good candidate for listeriosis prophylaxis. The population coverage was as high as 91.04% with a good immune response, indicating good antigen presentation with dendritic cells and production of memory cells. Conclusions: The findings of this study highlight the potential of the designed chimeric peptide construct as an effective subunit vaccine against Listeria, paving the way for future advances in preventive methods and vaccine design.

Original languageEnglish
Article number176
Number of pages12
JournalFrontiers in Bioscience - Landmark
Issue number5
Publication statusPublished - 9 May 2024


  • Food safety
  • Immunoinformatics
  • Listeria
  • Vaccine
  • Virulent proteins


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