TY - JOUR
T1 - Detailed kinetic model describing new oligosaccharides synthesis using different β-galactosidases
AU - Rodriguez-Fernandez, Maria
AU - Cardelle-Cobas, Alejandra
AU - Villamiel, Mar
AU - Banga, Julio R.
N1 - Funding Information:
This work was financed under the projects CONSOLIDER INGENIO 2010 FUNC-C-FOOD CSD2007-00063 from MICINN . A. Cardelle-Cobas thanks MEC for FPU grant. Authors M. Rodriguez-Fernandez and J.R. Banga acknowledge financial support from the EU ERASysBio and the Spanish Ministry of Science and Innovation (SYSMO project KOSMOBAC, ref. MEC GEN2006-27747-E/SYS and MICINN project MultiSysBio ref. DPI2008-06880-C03-02).
PY - 2011/5/20
Y1 - 2011/5/20
N2 - The production of prebiotic galactooligosaccharides (GOS) from lactose has been widely studied whereas the synthesis of new prebiotic oligosaccharides with improved properties as those derived from lactulose is receiving an increasing interest. Understanding the mechanism of enzymatic oligosaccharides synthesis from lactulose would help to improve the quality of the products in a rational way as well as to increase the production efficiency by optimally selecting the operating conditions. A detailed kinetic model describing the enzymatic transgalactosylation reaction during lactulose hydrolysis is presented here for the first time. The model was calibrated with the experimental data obtained in batch assays with two different β-galactosidases at various temperatures and concentrations of substrate. A complete system identification loop, including model selection, robust estimation of the parameters by means of a global optimization method and computation of confidence intervals was performed. The kinetic model showed a good agreement between experimental data and predictions for lactulose conversion and provided important insights into the mechanism of formation of new oligosaccharides with potential prebiotic properties.
AB - The production of prebiotic galactooligosaccharides (GOS) from lactose has been widely studied whereas the synthesis of new prebiotic oligosaccharides with improved properties as those derived from lactulose is receiving an increasing interest. Understanding the mechanism of enzymatic oligosaccharides synthesis from lactulose would help to improve the quality of the products in a rational way as well as to increase the production efficiency by optimally selecting the operating conditions. A detailed kinetic model describing the enzymatic transgalactosylation reaction during lactulose hydrolysis is presented here for the first time. The model was calibrated with the experimental data obtained in batch assays with two different β-galactosidases at various temperatures and concentrations of substrate. A complete system identification loop, including model selection, robust estimation of the parameters by means of a global optimization method and computation of confidence intervals was performed. The kinetic model showed a good agreement between experimental data and predictions for lactulose conversion and provided important insights into the mechanism of formation of new oligosaccharides with potential prebiotic properties.
KW - Identifiability analysis
KW - Kinetic models
KW - Lactulose
KW - Model selection
KW - Parameter estimation
KW - Transgalactosylation
UR - http://www.scopus.com/inward/record.url?scp=79955768786&partnerID=8YFLogxK
U2 - 10.1016/j.jbiotec.2011.03.012
DO - 10.1016/j.jbiotec.2011.03.012
M3 - Article
C2 - 21440015
AN - SCOPUS:79955768786
SN - 0168-1656
VL - 153
SP - 116
EP - 124
JO - Journal of Biotechnology
JF - Journal of Biotechnology
IS - 3-4
ER -