TY - JOUR
T1 - Development and validation of novel PCR primers for identification of plasmid-mediated colistin resistance (mcr) genes in various environmental settings
AU - Gorecki, Adrian
AU - Musialowski, Marcin
AU - Wolacewicz, Mikolaj
AU - Decewicz, Przemyslaw
AU - Ferreira, Catarina
AU - Vejmelkova, Dana
AU - Grzesiuk, Malgorzata
AU - Manaia, Célia M.
AU - Bartacek, Jan
AU - Dziewit, Lukasz
N1 - Funding Information:
This work was supported by the National Science Center (Poland) [grant number 2016/22/E/NZ8/00340 ] and the European Research Council under the European Union’s Horizon 2020 Research and Innovation through the project “Research platform on antibiotic resistance spread through wastewater treatment plants, REPARES” [grant number 857552 ]. This work was also partially (for CF and CMM) supported by the FEDER through the project “RISK.AR - Assessing the risks associated with environmental antibiotic-resistant bacteria: propagation and transmission to humans” ( PTDC/CTA-AMB/28196/2017 ) – Programa Operacional Competitividade e Internacionalização, and by National Funds from FCT – Fundação para a Ciência e a Tecnologia (Portugal), hosted by the CBQF FCT project UIDB/50016/2020 .
Funding Information:
This work was supported by the National Science Center (Poland) [grant number 2016/22/E/NZ8/00340] and the European Research Council under the European Union's Horizon 2020 Research and Innovation through the project “Research platform on antibiotic resistance spread through wastewater treatment plants, REPARES” [grant number 857552]. This work was also partially (for CF and CMM) supported by the FEDER through the project “RISK.AR - Assessing the risks associated with environmental antibiotic-resistant bacteria: propagation and transmission to humans” (PTDC/CTA-AMB/28196/2017) – Programa Operacional Competitividade e Internacionalização, and by National Funds from FCT – Fundação para a Ciência e a Tecnologia (Portugal), hosted by the CBQF FCT project UIDB/50016/2020.
Publisher Copyright:
© 2021 The Authors
PY - 2022/3/5
Y1 - 2022/3/5
N2 - Antibiotic resistance is considered one of the biggest threats to public health and has become a major concern for governments and international organizations. Combating this problem starts with improving global surveillance of antibiotic resistance genes (ARGs) and applying standardized protocols, both in a clinical and environmental context, in agreement with the One Health approach. Exceptional efforts should be directed to controlling ARGs conferring resistance to Critically Important Antimicrobials (CIA). In this study, a systematic literature review to synthesize data on the identification of mcr genes using a PCR technique was performed. Additionally, a novel set of PCR primers for mcr-1 – mcr-9 genes detection was proposed. The developed primers were in silico and experimentally validated by comparison with mcr-specific PCR primers reported in the literature. This validation, besides being a proof-of-concept for primers’ usefulness, provided insight into the distribution of mcr genes in municipal wastewater, clay and river sediments, glacier moraine, manure, seagulls and auks feces and daphnids from four countries. This analysis proved that commonly used primers may deliver false results, and some mcr genes may be overlooked in tested samples. Newly-developed PCR primers turned out to be relevant for the screening of mcr genes in various environments.
AB - Antibiotic resistance is considered one of the biggest threats to public health and has become a major concern for governments and international organizations. Combating this problem starts with improving global surveillance of antibiotic resistance genes (ARGs) and applying standardized protocols, both in a clinical and environmental context, in agreement with the One Health approach. Exceptional efforts should be directed to controlling ARGs conferring resistance to Critically Important Antimicrobials (CIA). In this study, a systematic literature review to synthesize data on the identification of mcr genes using a PCR technique was performed. Additionally, a novel set of PCR primers for mcr-1 – mcr-9 genes detection was proposed. The developed primers were in silico and experimentally validated by comparison with mcr-specific PCR primers reported in the literature. This validation, besides being a proof-of-concept for primers’ usefulness, provided insight into the distribution of mcr genes in municipal wastewater, clay and river sediments, glacier moraine, manure, seagulls and auks feces and daphnids from four countries. This analysis proved that commonly used primers may deliver false results, and some mcr genes may be overlooked in tested samples. Newly-developed PCR primers turned out to be relevant for the screening of mcr genes in various environments.
KW - Antibiotic resistance gene
KW - Colistin
KW - Mcr gene
KW - One health
KW - PCR primer
UR - http://www.scopus.com/inward/record.url?scp=85120827539&partnerID=8YFLogxK
U2 - 10.1016/j.jhazmat.2021.127936
DO - 10.1016/j.jhazmat.2021.127936
M3 - Article
C2 - 34883371
AN - SCOPUS:85120827539
SN - 0304-3894
VL - 425
JO - Journal of Hazardous Materials
JF - Journal of Hazardous Materials
M1 - 127936
ER -