TY - JOUR
T1 - Development of a flow method for the determination of phosphate in estuarine and freshwaters - comparison of flow cells in spectrophotometric sequential injection analysis
AU - Mesquita, Raquel B.R.
AU - Ferreira, M. Teresa S.O.B.
AU - Tóth, Ildikó V.
AU - Bordalo, Adriano A.
AU - McKelvie, Ian D.
AU - Rangel, António O.S.S.
PY - 2011/9/2
Y1 - 2011/9/2
N2 - A sequential injection system with dual analytical line was developed and applied in the comparison of two different detection systems viz; a conventional spectrophotometer with a commercial flow cell, and a multi-reflective flow cell coupled with a photometric detector under the same experimental conditions. The study was based on the spectrophotometric determination of phosphate using the molybdenum-blue chemistry. The two alternative flow cells were compared in terms of their response to variation of sample salinity, susceptibility to interferences and to refractive index changes. The developed method was applied to the determination of phosphate in natural waters (estuarine, river, well and ground waters). The achieved detection limit (0.007μM PO43-) is consistent with the requirement of the target water samples, and a wide quantification range (0.024-9.5μM) was achieved using both detection systems.
AB - A sequential injection system with dual analytical line was developed and applied in the comparison of two different detection systems viz; a conventional spectrophotometer with a commercial flow cell, and a multi-reflective flow cell coupled with a photometric detector under the same experimental conditions. The study was based on the spectrophotometric determination of phosphate using the molybdenum-blue chemistry. The two alternative flow cells were compared in terms of their response to variation of sample salinity, susceptibility to interferences and to refractive index changes. The developed method was applied to the determination of phosphate in natural waters (estuarine, river, well and ground waters). The achieved detection limit (0.007μM PO43-) is consistent with the requirement of the target water samples, and a wide quantification range (0.024-9.5μM) was achieved using both detection systems.
KW - Estuarine and river water
KW - Multi-reflective flow cell
KW - Phosphate determination
KW - Sequential injection
KW - Spectrophotometry
UR - http://www.scopus.com/inward/record.url?scp=79960340059&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2011.06.002
DO - 10.1016/j.aca.2011.06.002
M3 - Article
C2 - 21763803
AN - SCOPUS:79960340059
SN - 0003-2670
VL - 701
SP - 15
EP - 22
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -