Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218

Clara Sousa; Liliana Silva; Filipa Grosso; João Lopes; Luísa Peixe

doi:10.1016/j.jphotobiol.2014.02.015

Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218

Clara Sousa^*, Liliana Silva, Filipa Grosso, João Lopes, Luísa Peixe

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

37 Citations (Scopus)

Abstract

In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n = 148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.

Original language	English
Pages (from-to)	108-114
Number of pages	7
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	133
DOIs	https://doi.org/10.1016/j.jphotobiol.2014.02.015
Publication status	Published - 5 Apr 2014
Externally published	Yes

Keywords

Bacteria
Chemometry
Discriminant analysis
Epidemiology
FTIR
Spectroscopy
Typing

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10.1016/j.jphotobiol.2014.02.015

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title = "Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218",

abstract = "In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n = 148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.",

keywords = "Bacteria, Chemometry, Discriminant analysis, Epidemiology, FTIR, Spectroscopy, Typing",

author = "Clara Sousa and Liliana Silva and Filipa Grosso and Jo{\~a}o Lopes and Lu{\'i}sa Peixe",

note = "Funding Information: This study was supported by FCT Grants ( PEst-C/EQB/LA0006/2011 , POCI/AMB/61814/2004-FSE/FEDER ). Clara Sousa was supported by a post-doctoral grant from FCT, Ministry of Education and Science ( SFRH/BPD/70548/2010 ). Liliana Silva is a PhD student from FCT, Ministry of Education and Science ( SFRH/BD/88028/2012 ). The authors wish to thank L. Poirel, H. Seifert, P. Visca, J. Hrab{\'a}k and M. Asensi, G. Donelli and I. Goic-Barisi for kindly providing some of the isolates included in the study.",

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AU - Silva, Liliana

AU - Grosso, Filipa

AU - Lopes, João

AU - Peixe, Luísa

N1 - Funding Information: This study was supported by FCT Grants ( PEst-C/EQB/LA0006/2011 , POCI/AMB/61814/2004-FSE/FEDER ). Clara Sousa was supported by a post-doctoral grant from FCT, Ministry of Education and Science ( SFRH/BPD/70548/2010 ). Liliana Silva is a PhD student from FCT, Ministry of Education and Science ( SFRH/BD/88028/2012 ). The authors wish to thank L. Poirel, H. Seifert, P. Visca, J. Hrabák and M. Asensi, G. Donelli and I. Goic-Barisi for kindly providing some of the isolates included in the study.

PY - 2014/4/5

Y1 - 2014/4/5

N2 - In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n = 148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.

AB - In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n = 148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.

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KW - Chemometry

KW - Discriminant analysis

KW - Epidemiology

KW - FTIR

KW - Spectroscopy

KW - Typing

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Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218

Abstract

Keywords

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