Development of a FTIR-ATR based model for typing clinically relevant Acinetobacter baumannii clones belonging to ST98, ST103, ST208 and ST218

Clara Sousa*, Liliana Silva, Filipa Grosso, João Lopes, Luísa Peixe

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

In this work we developed and validated a FTIR-ATR (Fourier transform infrared spectroscopy with attenuated total reflectance) based model for typing Acinetobacter baumannii clinical isolates belonging to ST98, ST208 and ST218 included into the worldwide spread clonal complex (CC) 92 and ST103. FTIR-ATR spectra of seventy-seven previously characterized isolates (Multi Locus Sequence Type-MLST, Pulsed-Field Gel Electrophoresis-PFGE and carbapenem-hydrolyzing class D β-lactamase-CHDL) were acquired and modeled by partial least squares discriminant analysis (PLSDA). The model was tested and successfully validated with a diverse collection of isolates (n = 148) recovered from different countries and periods of time belonging to modeled and non-modeled STs.
Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalJournal of Photochemistry and Photobiology B: Biology
Volume133
DOIs
Publication statusPublished - 5 Apr 2014
Externally publishedYes

Keywords

  • Bacteria
  • Chemometry
  • Discriminant analysis
  • Epidemiology
  • FTIR
  • Spectroscopy
  • Typing

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