Development of a low pressure chromatographic flow system for monitoring the biodegradation of ofloxacin and ciprofloxacin

Inês C. Santos, Raquel B. R. Mesquita, Catarina L. Amorim, Paula M. L. Castro, António O. S. S. Rangel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


In this work, we propose a simple low pressure chromatography method with a high throughput for monitoring the biodegradation of fluoroquinolones. Fluoroquinolones are a class of antibiotics that have been accumulating in the environment as a consequence of their release from different sources, namely hospital waste. It has been found that wastewater treatment plants are not able to completely remove this type of micro-pollutants and so, alternative solutions are necessary. Some biodegradation studies have been done but the HPLC-based methodologies used to monitor the degradation process usually require high cost instrumentation such as analytical columns and detectors. For this reason, a 1 cm monolithic column was coupled to a flow injection system and used for the simultaneous quantification of ofloxacin and ciprofloxacin with UV detection at 295 and 275 nm, respectively. LODs of 0.5 and 0.29 mg L-1 for ofloxacin and ciprofloxacin were obtained, respectively, with repeatability within the range of 2-10%. The developed method was successfully applied to monitor the biodegradation of ofloxacin and ciprofloxacin by the strain Labrys portucalensis F11. The results proved that the low pressure chromatography method is a simpler, cheaper, and faster alternative to monitor biodegradation studies.
Original languageEnglish
Pages (from-to)5457-5465
Number of pages9
JournalAnalytical Methods
Issue number27
Publication statusPublished - 21 Jul 2016


Dive into the research topics of 'Development of a low pressure chromatographic flow system for monitoring the biodegradation of ofloxacin and ciprofloxacin'. Together they form a unique fingerprint.

Cite this