Disengaging the Smc3/kleisin interface releases cohesin from Drosophila chromosomes during interphase and mitosis

Christian S. Eichinger, Alexander Kurze, Raquel A. Oliveira, Kim Nasmyth*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

64 Citations (Scopus)

Abstract

Cohesin's Smc1, Smc3, and kleisin subunits create a tripartite ring within which sister DNAs are entrapped. Evidence suggests that DNA enters through a gate created by transient dissociation of the Smc1/3 interface. Release at the onset of anaphase is triggered by proteolytic cleavage of kleisin. Less well understood is the mechanism of release at other stages of the cell cycle, in particular during prophase when most cohesin dissociates from chromosome arms in a process dependent on the regulatory subunit Wapl. We show here that Wapl-dependent release from salivary gland polytene chromosomes during interphase and from neuroblast chromosome arms during prophase is blocked by translational fusion of Smc3's C-terminus to kleisin's N-terminus. Our findings imply that proteolysis-independent release of cohesin from chromatin is mediated by Wapl-dependent escape of DNAs through a gate created by transient dissociation of the Smc3/kleisin interface. Thus, cohesin's DNA entry and exit gates are distinct.

Original languageEnglish
Pages (from-to)656-665
Number of pages10
JournalEMBO Journal
Volume32
Issue number5
DOIs
Publication statusPublished - 6 Mar 2013
Externally publishedYes

Keywords

  • cohesin
  • Drosophila melanogaster
  • prophase pathway
  • sister chromatid cohesion
  • Wapl

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