Abstract
Most of the fast in vitro assays proposed to determine the antioxidant capacity of a compound/extract lack either biological context or employ complex protocols. Therefore, the present work proposes the improvement of an agarose gel DNA electrophoresis in order to allow for a quantitative estimation of the antioxidant capacity of pure phenolic compounds as well as of a phenolic rich extract, while also considering their possible pro-oxidant effects. The result obtained demonstrated that the proposed method allowed for the evaluation of the protection of DNA oxidation [in the presence of hydrogen peroxide (H2O2) and an H2O2/iron (III) chloride (FeCl3) systems] as well as for the observation of pro-oxidant activities, with the measurements registering interclass correlation coefficients above 0.9. Moreover, this method allowed for the characterization of the antioxidant capacity of a blueberry extract while demonstrating that it had no perceived pro-oxidant effect.
Original language | English |
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Pages (from-to) | 45-51 |
Number of pages | 7 |
Journal | Food Chemistry |
Volume | 233 |
DOIs | |
Publication status | Published - 15 Oct 2017 |
Keywords
- Antioxidant assays
- Ascorbic acid
- Blueberry extract
- DNA
- Gallic acid
- Phenolic compounds
- Pro-oxidant assays