TY - JOUR
T1 - DNA-based sensor against nitrite oxide radical
T2 - evaluation of total antioxidant capacity in beverages
AU - Cruz, Diana
AU - Barroso, M. Fátima
AU - Ramalhosa, Maria João
AU - Coelho, Andreia
AU - Silva, Hélder da
AU - Duarte, Abel J.
AU - González-García, Maria Begoña
AU - Carvalho, Ana P.
AU - Delerue-Matos, C.
N1 - Funding Information:
M.F. Barroso is grateful to Fundação para a Ciência e a Tecnologia (FCT) for the post-doc fellowship ( SFRH/BPD/78845/2011 ), financed by POPH–QREN–Tipologia 4.1–Formação Avançada, subsidized by Fundo Social Europeu and Ministério da Ciência, Tecnologia e Ensino Superior. This work received financial support from the European Union (FEDER funds through COMPETE) and National Funds (FCT) through project UID/QUI/50006/2013 . Author Carvalho acknowledge FEDER funds through CCDR-N , in the scope of project Operação NORTE-07-0124-FEDER-000069 — Ciência do Alimento.
Publisher Copyright:
© 2015 Elsevier B.V. All rights reserved.
PY - 2016/2/15
Y1 - 2016/2/15
N2 - Reactive nitrogen and oxygen species are produced in cells and play an important role in the oxidative metabolism; when in abnormal concentrations, they are able to induce oxidative damage on biomolecules, namely in DNA. In this paper it is described, an electrochemical DNA-based sensor against NO radical developed for total antioxidant capacity (TAC) evaluation. The sensor consisted on dA20 (adenine-rich oligonucleotide) physically adsorbed into carbon paste electrode (CPE). When this dA20-CPE was damaged, by immersion in a freshly generated NO radical, a protective effect onto dA20 was observed in the presence of antioxidants (ascorbic, gallic, caffeic, p-coumaric acids). Electrochemical studies were performed through square wave voltammetry. The construction of the sensor is simple, fast and the results indicated that the DNA-based sensor is suitable, accurate, and can be used to the assessment of TAC in commercial samples of juices.
AB - Reactive nitrogen and oxygen species are produced in cells and play an important role in the oxidative metabolism; when in abnormal concentrations, they are able to induce oxidative damage on biomolecules, namely in DNA. In this paper it is described, an electrochemical DNA-based sensor against NO radical developed for total antioxidant capacity (TAC) evaluation. The sensor consisted on dA20 (adenine-rich oligonucleotide) physically adsorbed into carbon paste electrode (CPE). When this dA20-CPE was damaged, by immersion in a freshly generated NO radical, a protective effect onto dA20 was observed in the presence of antioxidants (ascorbic, gallic, caffeic, p-coumaric acids). Electrochemical studies were performed through square wave voltammetry. The construction of the sensor is simple, fast and the results indicated that the DNA-based sensor is suitable, accurate, and can be used to the assessment of TAC in commercial samples of juices.
KW - Commercial juice samples
KW - DNA-based sensor
KW - Electrochemistry
KW - Reactive nitrogen and oxygen species
KW - Total antioxidant capacity (TAC)
UR - http://www.scopus.com/inward/record.url?scp=84953775268&partnerID=8YFLogxK
U2 - 10.1016/j.jelechem.2015.12.045
DO - 10.1016/j.jelechem.2015.12.045
M3 - Article
AN - SCOPUS:84953775268
SN - 1572-6657
VL - 763
SP - 110
EP - 115
JO - Journal of Electroanalytical Chemistry
JF - Journal of Electroanalytical Chemistry
ER -
