Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci

Janusz Błaszkowski; Piotr Niezgoda; Szymon Zubek; Edward Meller; Paweł Milczarski; Monika Malicka; Bruno Tomio Goto; Gabriela Woźniak; Helena Moreira; Franco Magurno

doi:10.1007/s11557-020-01659-4

Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci

Janusz Błaszkowski^*, Piotr Niezgoda, Szymon Zubek, Edward Meller, Paweł Milczarski, Monika Malicka, Bruno Tomio Goto, Gabriela Woźniak, Helena Moreira, Franco Magurno

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Examination of morphological characters of two arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), which produced clusters with glomoid spores in single-species cultures, suggested that they were undescribed species, one belonging to the genera Dominikia, Microdominikia or Kamienskia, and one to the genus Glomus. Phylogenetic analyses of the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and large subunit (28S) (= 18S-ITS-28S) region, of the largest subunit of RNA polymerase II (RPB1) gene, as well as of concatenated sequences of these two loci (18S-ITS-28S + RPB1) confirmed our hypotheses and proved that both fungi were new species, here described as Dominikia bonfanteae sp. nov. and Glomus atlanticum sp. nov., respectively. In nature, D. bonfanteae was found in a plant community on the shore of Kokotek pond II (Lubliniec in the Silesian Upland, Poland). Glomus atlanticum originated from the Dunes of Aguda Park (Northern Portugal). The phylogenetic position of these new species and their close family relatives within the Glomeraceae were compared and discussed depending on the sequence alignment analysed. This paper for the first time shows the phylogeny of AMF reconstructed from analyses of two concatenated unlinked loci: 18S-ITS-28S plus RPB1. The partial RPB1 gene of D. bonfanteae was amplified using newly designed primers.

Original language	English
Pages (from-to)	131-148
Number of pages	18
Journal	Mycological Progress
Volume	20
Issue number	2
DOIs	https://doi.org/10.1007/s11557-020-01659-4
Publication status	Published - Feb 2021

Keywords

Arbuscular mycorrhizal fungi
Morphology
Molecular phylogeny
RPB1
18S-ITS-28S nrDNA
18S-ITS-28S + RPB1

Access to Document

10.1007/s11557-020-01659-4

Cite this

Błaszkowski, J., Niezgoda, P., Zubek, S., Meller, E., Milczarski, P., Malicka, M., Goto, B. T., Woźniak, G., Moreira, H., & Magurno, F. (2021). Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci. Mycological Progress, 20(2), 131-148. https://doi.org/10.1007/s11557-020-01659-4

@article{cd6728604ce34def94bd049664c14bb8,

title = "Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci",

abstract = "Examination of morphological characters of two arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), which produced clusters with glomoid spores in single-species cultures, suggested that they were undescribed species, one belonging to the genera Dominikia, Microdominikia or Kamienskia, and one to the genus Glomus. Phylogenetic analyses of the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and large subunit (28S) (= 18S-ITS-28S) region, of the largest subunit of RNA polymerase II (RPB1) gene, as well as of concatenated sequences of these two loci (18S-ITS-28S + RPB1) confirmed our hypotheses and proved that both fungi were new species, here described as Dominikia bonfanteae sp. nov. and Glomus atlanticum sp. nov., respectively. In nature, D. bonfanteae was found in a plant community on the shore of Kokotek pond II (Lubliniec in the Silesian Upland, Poland). Glomus atlanticum originated from the Dunes of Aguda Park (Northern Portugal). The phylogenetic position of these new species and their close family relatives within the Glomeraceae were compared and discussed depending on the sequence alignment analysed. This paper for the first time shows the phylogeny of AMF reconstructed from analyses of two concatenated unlinked loci: 18S-ITS-28S plus RPB1. The partial RPB1 gene of D. bonfanteae was amplified using newly designed primers.",

keywords = "Arbuscular mycorrhizal fungi, Morphology, Molecular phylogeny, RPB1, 18S-ITS-28S nrDNA, 18S-ITS-28S + RPB1",

author = "Janusz B{\l}aszkowski and Piotr Niezgoda and Szymon Zubek and Edward Meller and Pawe{\l} Milczarski and Monika Malicka and Goto, {Bruno Tomio} and Gabriela Wo{\'z}niak and Helena Moreira and Franco Magurno",

note = "Funding Information: To Piotr Niezgoda by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, to Bruno Tomio Goto by Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico, proc. 408011-2016-5), to Szymon Zubek by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. Funding Information: Part of this work was supported by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, as well as by Conselho Nacional de Desenvolvimento Cient{\'i}fico e Tecnol{\'o}gico (CNPq) that provided research grants to BT Goto and financial support in the project “Sporocarpic species of arbuscular mycorrhizal fungi (Glomeromycota): taxonomy, systematic and evolution” (proc.408011-2016-5). We also thank Universidade Federal do Rio Grande do Norte for covering the costs of B.T. Goto{\textquoteright}s stay as collaborative research in West Pomeranian University of Technology in Szczecin in the period from December 2019 to January 2020. The research was funded, in part, by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. The authors also thank two anonymous reviewers and the section editor, Dr. Marco Tines, for their constructive comments. Funding Information: Part of this work was supported by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, as well as by Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) that provided research grants to BT Goto and financial support in the project ?Sporocarpic species of arbuscular mycorrhizal fungi (Glomeromycota): taxonomy, systematic and evolution? (proc.408011-2016-5). We also thank Universidade Federal do Rio Grande do Norte for covering the costs of B.T. Goto?s stay as collaborative research in West Pomeranian University of Technology in Szczecin in the period from December 2019 to January 2020. The research was funded, in part, by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. The authors also thank two anonymous reviewers and the section editor, Dr. Marco Tines, for their constructive comments. Publisher Copyright: {\textcopyright} 2021, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature.",

year = "2021",

month = feb,

doi = "10.1007/s11557-020-01659-4",

language = "English",

volume = "20",

pages = "131--148",

journal = "Mycological Progress",

issn = "1617-416X",

publisher = "Springer Verlag",

number = "2",

}

Błaszkowski, J, Niezgoda, P, Zubek, S, Meller, E, Milczarski, P, Malicka, M, Goto, BT, Woźniak, G, Moreira, H & Magurno, F 2021, 'Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci', Mycological Progress, vol. 20, no. 2, pp. 131-148. https://doi.org/10.1007/s11557-020-01659-4

TY - JOUR

T1 - Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci

AU - Błaszkowski, Janusz

AU - Niezgoda, Piotr

AU - Zubek, Szymon

AU - Meller, Edward

AU - Milczarski, Paweł

AU - Malicka, Monika

AU - Goto, Bruno Tomio

AU - Woźniak, Gabriela

AU - Moreira, Helena

AU - Magurno, Franco

N1 - Funding Information: To Piotr Niezgoda by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, to Bruno Tomio Goto by Conselho Nacional de Desenvolvimento Científico e Tecnológico, proc. 408011-2016-5), to Szymon Zubek by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. Funding Information: Part of this work was supported by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, as well as by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) that provided research grants to BT Goto and financial support in the project “Sporocarpic species of arbuscular mycorrhizal fungi (Glomeromycota): taxonomy, systematic and evolution” (proc.408011-2016-5). We also thank Universidade Federal do Rio Grande do Norte for covering the costs of B.T. Goto’s stay as collaborative research in West Pomeranian University of Technology in Szczecin in the period from December 2019 to January 2020. The research was funded, in part, by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. The authors also thank two anonymous reviewers and the section editor, Dr. Marco Tines, for their constructive comments. Funding Information: Part of this work was supported by Polish National Centre of Science, grant no. 2020/37/N/NZ9/00509, as well as by Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq) that provided research grants to BT Goto and financial support in the project ?Sporocarpic species of arbuscular mycorrhizal fungi (Glomeromycota): taxonomy, systematic and evolution? (proc.408011-2016-5). We also thank Universidade Federal do Rio Grande do Norte for covering the costs of B.T. Goto?s stay as collaborative research in West Pomeranian University of Technology in Szczecin in the period from December 2019 to January 2020. The research was funded, in part, by the Institute of Botany at the Jagiellonian University, project no. N18/DBS/000002. The authors also thank two anonymous reviewers and the section editor, Dr. Marco Tines, for their constructive comments. Publisher Copyright: © 2021, German Mycological Society and Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2021/2

Y1 - 2021/2

N2 - Examination of morphological characters of two arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), which produced clusters with glomoid spores in single-species cultures, suggested that they were undescribed species, one belonging to the genera Dominikia, Microdominikia or Kamienskia, and one to the genus Glomus. Phylogenetic analyses of the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and large subunit (28S) (= 18S-ITS-28S) region, of the largest subunit of RNA polymerase II (RPB1) gene, as well as of concatenated sequences of these two loci (18S-ITS-28S + RPB1) confirmed our hypotheses and proved that both fungi were new species, here described as Dominikia bonfanteae sp. nov. and Glomus atlanticum sp. nov., respectively. In nature, D. bonfanteae was found in a plant community on the shore of Kokotek pond II (Lubliniec in the Silesian Upland, Poland). Glomus atlanticum originated from the Dunes of Aguda Park (Northern Portugal). The phylogenetic position of these new species and their close family relatives within the Glomeraceae were compared and discussed depending on the sequence alignment analysed. This paper for the first time shows the phylogeny of AMF reconstructed from analyses of two concatenated unlinked loci: 18S-ITS-28S plus RPB1. The partial RPB1 gene of D. bonfanteae was amplified using newly designed primers.

AB - Examination of morphological characters of two arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), which produced clusters with glomoid spores in single-species cultures, suggested that they were undescribed species, one belonging to the genera Dominikia, Microdominikia or Kamienskia, and one to the genus Glomus. Phylogenetic analyses of the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and large subunit (28S) (= 18S-ITS-28S) region, of the largest subunit of RNA polymerase II (RPB1) gene, as well as of concatenated sequences of these two loci (18S-ITS-28S + RPB1) confirmed our hypotheses and proved that both fungi were new species, here described as Dominikia bonfanteae sp. nov. and Glomus atlanticum sp. nov., respectively. In nature, D. bonfanteae was found in a plant community on the shore of Kokotek pond II (Lubliniec in the Silesian Upland, Poland). Glomus atlanticum originated from the Dunes of Aguda Park (Northern Portugal). The phylogenetic position of these new species and their close family relatives within the Glomeraceae were compared and discussed depending on the sequence alignment analysed. This paper for the first time shows the phylogeny of AMF reconstructed from analyses of two concatenated unlinked loci: 18S-ITS-28S plus RPB1. The partial RPB1 gene of D. bonfanteae was amplified using newly designed primers.

KW - Arbuscular mycorrhizal fungi

KW - Morphology

KW - Molecular phylogeny

KW - RPB1

KW - 18S-ITS-28S nrDNA

KW - 18S-ITS-28S + RPB1

UR - http://www.scopus.com/inward/record.url?scp=85100321789&partnerID=8YFLogxK

U2 - 10.1007/s11557-020-01659-4

DO - 10.1007/s11557-020-01659-4

M3 - Article

AN - SCOPUS:85100321789

SN - 1617-416X

VL - 20

SP - 131

EP - 148

JO - Mycological Progress

JF - Mycological Progress

IS - 2

ER -

Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

CBQF Multianual Funding: Centre for Biotechnology and Fine Chemistry

Cite this

Dominikia bonfanteae and glomus atlanticum, two new species in the glomeraceae (phylum glomeromycota) with molecular phylogenies reconstructed from two unlinked loci

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

CBQF Multianual Funding: Centre for Biotechnology and Fine Chemistry

Cite this