Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine

Maria Michela Salvatore, Carina Félix, Fernanda Lima, Vanessa Ferreira, Ana Sofia Duarte, Francesco Salvatore, Artur Alves, Ana Cristina Esteves, Anna Andolfi

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6 Citations (Scopus)

Abstract

The effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine that hold a different degree of virulence to the host plant (LA-SOL3 (more virulent), LA-SV1 (less virulent)) was investigated. The culture filtrates and crude extracts from the two strains grown in the presence and absence of 10 mM of GABA were tested for phytotoxicity on tomato plant cuttings and leaves, respectively. Considering the opportunistic nature of this fungus for humans, crude extracts were also tested for cytotoxicity on mammalian cell lines. We found that culture filtrates and crude extracts have a decreased toxicity in the presence of GABA. Metabolomic analysis, conducted on both strains at both growth conditions, revealed the production of several compounds, such as indole-3-carboxylic acid (ICA, which is the main compound produced by L. theobromae), 3-indolecarboxyaldehyde, (3R,4S)-botryodiplodin, (R)-mellein. Finally, data demonstrate that GABA both induces a decrease in the amount of ICA, and a diversification of the metabolites produced by L. theobromae.
Original languageEnglish
Article number3833
JournalMolecules (Basel, Switzerland)
Volume25
Issue number17
DOIs
Publication statusPublished - 23 Aug 2020

Keywords

  • Grapevine
  • Indole-3-carboxylic acid
  • Lasiodiplodia theobromae
  • Plant diseases
  • Secondary metabolites

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