Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine

Maria Michela Salvatore; Carina Félix; Fernanda Lima; Vanessa Ferreira; Ana Sofia Duarte; Francesco Salvatore; Artur Alves; Ana Cristina Esteves; Anna Andolfi

doi:10.3390/molecules25173833

Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine

Maria Michela Salvatore^*, Carina Félix^*, Fernanda Lima^*, Vanessa Ferreira^*, Ana Sofia Duarte^*, Francesco Salvatore^*, Artur Alves^*, Ana Cristina Esteves^*, Anna Andolfi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine that hold a different degree of virulence to the host plant (LA-SOL3 (more virulent), LA-SV1 (less virulent)) was investigated. The culture filtrates and crude extracts from the two strains grown in the presence and absence of 10 mM of GABA were tested for phytotoxicity on tomato plant cuttings and leaves, respectively. Considering the opportunistic nature of this fungus for humans, crude extracts were also tested for cytotoxicity on mammalian cell lines. We found that culture filtrates and crude extracts have a decreased toxicity in the presence of GABA. Metabolomic analysis, conducted on both strains at both growth conditions, revealed the production of several compounds, such as indole-3-carboxylic acid (ICA, which is the main compound produced by L. theobromae), 3-indolecarboxyaldehyde, (3R,4S)-botryodiplodin, (R)-mellein. Finally, data demonstrate that GABA both induces a decrease in the amount of ICA, and a diversification of the metabolites produced by L. theobromae.

Original language	English
Article number	3833
Number of pages	17
Journal	Molecules (Basel, Switzerland)
Volume	25
Issue number	17
DOIs	https://doi.org/10.3390/molecules25173833
Publication status	Published - 23 Aug 2020

Keywords

Grapevine
Indole-3-carboxylic acid
Lasiodiplodia theobromae
Plant diseases
Secondary metabolites

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10.3390/molecules25173833Licence: CC BY

20274541Final published version, 3.87 MBLicence: CC BY

http://hdl.handle.net/10400.14/32269Licence: CC BY

Cite this

@article{5084fdc0dd9546688b0af88c4f197449,

title = "Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine",

abstract = "The effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine that hold a different degree of virulence to the host plant (LA-SOL3 (more virulent), LA-SV1 (less virulent)) was investigated. The culture filtrates and crude extracts from the two strains grown in the presence and absence of 10 mM of GABA were tested for phytotoxicity on tomato plant cuttings and leaves, respectively. Considering the opportunistic nature of this fungus for humans, crude extracts were also tested for cytotoxicity on mammalian cell lines. We found that culture filtrates and crude extracts have a decreased toxicity in the presence of GABA. Metabolomic analysis, conducted on both strains at both growth conditions, revealed the production of several compounds, such as indole-3-carboxylic acid (ICA, which is the main compound produced by L. theobromae), 3-indolecarboxyaldehyde, (3R,4S)-botryodiplodin, (R)-mellein. Finally, data demonstrate that GABA both induces a decrease in the amount of ICA, and a diversification of the metabolites produced by L. theobromae.",

keywords = "Grapevine, Indole-3-carboxylic acid, Lasiodiplodia theobromae, Plant diseases, Secondary metabolites",

author = "Salvatore, {Maria Michela} and Carina F{\'e}lix and Fernanda Lima and Vanessa Ferreira and Duarte, {Ana Sofia} and Francesco Salvatore and Artur Alves and Esteves, {Ana Cristina} and Anna Andolfi",

note = "Funding Information: This research was funded by FEDER funding through COMPETE program (POCI-01-0145-FEDER-016788) and Programa Operacional Regional de Lisboa-POR Lisboa (LISBOA-01-0145-FEDER-016788) and by national funding through FCT within the research project ALIEN (PTDC/AGR-PRO/2183/2014). CESAM received financial support from FCT/MCTES (UIDP/50017/2020+UIDB/50017/2020), through national funds. AC Esteves and AS Duarte (CEECINST/00137/2018) were funded by FCT and UCP for the CEEC institutional financing (CEECINST/00137/2018). Funding Information: Funding: This research was funded by FEDER funding through COMPETE program (POCI-01-0145-FEDER-016788) and Programa Operacional Regional de Lisboa-POR Lisboa (LISBOA-01-0145-FEDER-016788) and by national funding through FCT within the research project ALIEN (PTDC/AGR-PRO/2183/2014). CESAM received financial support from FCT/MCTES (UIDP/50017/2020+UIDB/50017/2020), through national funds. AC Esteves and AS Duarte (CEECINST/00137/2018) were funded by FCT and UCP for the CEEC institutional financing (CEECINST/00137/2018). Publisher Copyright: {\textcopyright} 2020 by the authors.",

year = "2020",

month = aug,

day = "23",

doi = "10.3390/molecules25173833",

language = "English",

volume = "25",

journal = "Molecules (Basel, Switzerland)",

issn = "1420-3049",

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T1 - Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine

AU - Salvatore, Maria Michela

AU - Félix, Carina

AU - Lima, Fernanda

AU - Ferreira, Vanessa

AU - Duarte, Ana Sofia

AU - Salvatore, Francesco

AU - Alves, Artur

AU - Esteves, Ana Cristina

AU - Andolfi, Anna

N1 - Funding Information: This research was funded by FEDER funding through COMPETE program (POCI-01-0145-FEDER-016788) and Programa Operacional Regional de Lisboa-POR Lisboa (LISBOA-01-0145-FEDER-016788) and by national funding through FCT within the research project ALIEN (PTDC/AGR-PRO/2183/2014). CESAM received financial support from FCT/MCTES (UIDP/50017/2020+UIDB/50017/2020), through national funds. AC Esteves and AS Duarte (CEECINST/00137/2018) were funded by FCT and UCP for the CEEC institutional financing (CEECINST/00137/2018). Funding Information: Funding: This research was funded by FEDER funding through COMPETE program (POCI-01-0145-FEDER-016788) and Programa Operacional Regional de Lisboa-POR Lisboa (LISBOA-01-0145-FEDER-016788) and by national funding through FCT within the research project ALIEN (PTDC/AGR-PRO/2183/2014). CESAM received financial support from FCT/MCTES (UIDP/50017/2020+UIDB/50017/2020), through national funds. AC Esteves and AS Duarte (CEECINST/00137/2018) were funded by FCT and UCP for the CEEC institutional financing (CEECINST/00137/2018). Publisher Copyright: © 2020 by the authors.

PY - 2020/8/23

Y1 - 2020/8/23

N2 - The effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine that hold a different degree of virulence to the host plant (LA-SOL3 (more virulent), LA-SV1 (less virulent)) was investigated. The culture filtrates and crude extracts from the two strains grown in the presence and absence of 10 mM of GABA were tested for phytotoxicity on tomato plant cuttings and leaves, respectively. Considering the opportunistic nature of this fungus for humans, crude extracts were also tested for cytotoxicity on mammalian cell lines. We found that culture filtrates and crude extracts have a decreased toxicity in the presence of GABA. Metabolomic analysis, conducted on both strains at both growth conditions, revealed the production of several compounds, such as indole-3-carboxylic acid (ICA, which is the main compound produced by L. theobromae), 3-indolecarboxyaldehyde, (3R,4S)-botryodiplodin, (R)-mellein. Finally, data demonstrate that GABA both induces a decrease in the amount of ICA, and a diversification of the metabolites produced by L. theobromae.

AB - The effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine that hold a different degree of virulence to the host plant (LA-SOL3 (more virulent), LA-SV1 (less virulent)) was investigated. The culture filtrates and crude extracts from the two strains grown in the presence and absence of 10 mM of GABA were tested for phytotoxicity on tomato plant cuttings and leaves, respectively. Considering the opportunistic nature of this fungus for humans, crude extracts were also tested for cytotoxicity on mammalian cell lines. We found that culture filtrates and crude extracts have a decreased toxicity in the presence of GABA. Metabolomic analysis, conducted on both strains at both growth conditions, revealed the production of several compounds, such as indole-3-carboxylic acid (ICA, which is the main compound produced by L. theobromae), 3-indolecarboxyaldehyde, (3R,4S)-botryodiplodin, (R)-mellein. Finally, data demonstrate that GABA both induces a decrease in the amount of ICA, and a diversification of the metabolites produced by L. theobromae.

KW - Grapevine

KW - Indole-3-carboxylic acid

KW - Lasiodiplodia theobromae

KW - Plant diseases

KW - Secondary metabolites

UR - http://www.scopus.com/inward/record.url?scp=85089923492&partnerID=8YFLogxK

U2 - 10.3390/molecules25173833

DO - 10.3390/molecules25173833

M3 - Article

C2 - 32842542

AN - SCOPUS:85089923492

SN - 1420-3049

VL - 25

JO - Molecules (Basel, Switzerland)

JF - Molecules (Basel, Switzerland)

IS - 17

M1 - 3833

ER -

Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine

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Keywords

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CIIS: Center for Interdisciplinary Research in Health

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Effect of γ-aminobutyric acid (GABA) on the metabolome of two strains of Lasiodiplodia theobromae isolated from grapevine

Abstract

Keywords

Access to Document

Other files and links

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CIIS: Center for Interdisciplinary Research in Health

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