TY - JOUR
T1 - Effect of γ-lactones and γ-lactams compounds on streptococcus mutans biofilms
AU - Sordi, Mariane Beatriz
AU - Moreira, Thaís Altoé
AU - Montero, Juan Felipe Dumes
AU - Barbosa, Luis Cláudio
AU - Benfatti, César Augusto Magalhães
AU - Magini, Ricardo de Souza
AU - Pimenta, Andréa de Lima
AU - de Souza, Júlio César Matias
N1 - Publisher Copyright:
© 2018, Faculdade de Odontologia de Bauru. All rights reserved.
PY - 2018
Y1 - 2018
N2 - Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective: To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods: We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 µg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results: Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions: Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.
AB - Considering oral diseases, antibiofilm compounds can decrease the accumulation of pathogenic species such as Streptococcus mutans at micro-areas of teeth, dental restorations or implant-supported prostheses. Objective: To assess the effect of thirteen different novel lactam-based compounds on the inhibition of S. mutans biofilm formation. Material and methods: We synthesized compounds based on γ-lactones analogues from rubrolides by a mucochloric acid process and converted them into their corresponding γ-hydroxy-γ-lactams by a reaction with isobutylamine and propylamine. Compounds concentrations ranging from 0.17 up to 87.5 µg mL-1 were tested against S. mutans. We diluted the exponential cultures in TSB and incubated them (37°C) in the presence of different γ-lactones or γ-lactams dilutions. Afterwards, we measured the planktonic growth by optical density at 630 nm and therefore assessed the biofilm density by the crystal violet staining method. Results: Twelve compounds were active against biofilm formation, showing no effect on bacterial viability. Only one compound was inactive against both planktonic and biofilm growth. The highest biofilm inhibition (inhibition rate above 60%) was obtained for two compounds while three other compounds revealed an inhibition rate above 40%. Conclusions: Twelve of the thirteen compounds revealed effective inhibition of S. mutans biofilm formation, with eight of them showing a specific antibiofilm effect.
KW - Antimicrobial
KW - Biofilm
KW - Lactams
KW - Lactones
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=85042468045&partnerID=8YFLogxK
U2 - 10.1590/1678-7757-2017-0065
DO - 10.1590/1678-7757-2017-0065
M3 - Article
C2 - 29489934
AN - SCOPUS:85042468045
SN - 1678-7757
VL - 26
JO - Journal of Applied Oral Science
JF - Journal of Applied Oral Science
M1 - e20170065
ER -