Effect of chemical stress and ultraviolet radiation in the bacterial communities of zebrafish embryos

Jacinta M.M. Oliveira; Ana Rita Almeida; Tânia Pimentel; Thayres S. Andrade; Jorge F. Henriques; Amadeu M.V.M. Soares; Susana Loureiro; Newton C.M. Gomes; Inês Domingues

doi:10.1016/j.envpol.2015.10.039

Effect of chemical stress and ultraviolet radiation in the bacterial communities of zebrafish embryos

Jacinta M.M. Oliveira^*, Ana Rita Almeida, Tânia Pimentel, Thayres S. Andrade, Jorge F. Henriques, Amadeu M.V.M. Soares, Susana Loureiro, Newton C.M. Gomes, Inês Domingues

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

This study aimed to assess the effect of ultraviolet radiation (UVR) and chemical stress (triclosan-TCS; potassium dichromate-PD; prochloraz-PCZ) on bacterial communities of zebrafish (Danio rerio) embryos (ZEBC). Embryos were exposed to two UVR intensities and two chemical concentrations not causing mortality or any developmental effect (equivalent to the No-Observed-Effect Concentration-NOEC; NOEC diluted by 10-NOEC/10). Effects on ZEBC were evaluated using denaturing gradient gel electrophoresis (DGGE) and interpreted considering structure, richness and diversity. ZEBC were affected by both stressors even at concentrations/doses not affecting the host-organism (survival/development). Yet, some stress-tolerant bacterial groups were revealed. The structure of the ZEBC was always affected, mainly due to xenobiotic presence. Richness and diversity decreased after exposure to NOEC of PD. Interactive effects occurred for TCS and UVR. Aquatic microbiota imbalance might have repercussions for the host/aquatic system, particularly in a realistic scenario/climate change perspective therefore, future ecotoxicological models should consider xenobiotics interactions with UVR.

Original language	English
Pages (from-to)	626-636
Number of pages	11
Journal	Environmental Pollution
Volume	208
DOIs	https://doi.org/10.1016/j.envpol.2015.10.039
Publication status	Published - 1 Jan 2016
Externally published	Yes

Keywords

Chemical stress
DGGE
Danio rerio embryos
Microbiota
Ultraviolet radiation

Access to Document

10.1016/j.envpol.2015.10.039

Cite this

@article{2d760554696b474d86eff18ac0262eb1,

title = "Effect of chemical stress and ultraviolet radiation in the bacterial communities of zebrafish embryos",

abstract = "This study aimed to assess the effect of ultraviolet radiation (UVR) and chemical stress (triclosan-TCS; potassium dichromate-PD; prochloraz-PCZ) on bacterial communities of zebrafish (Danio rerio) embryos (ZEBC). Embryos were exposed to two UVR intensities and two chemical concentrations not causing mortality or any developmental effect (equivalent to the No-Observed-Effect Concentration-NOEC; NOEC diluted by 10-NOEC/10). Effects on ZEBC were evaluated using denaturing gradient gel electrophoresis (DGGE) and interpreted considering structure, richness and diversity. ZEBC were affected by both stressors even at concentrations/doses not affecting the host-organism (survival/development). Yet, some stress-tolerant bacterial groups were revealed. The structure of the ZEBC was always affected, mainly due to xenobiotic presence. Richness and diversity decreased after exposure to NOEC of PD. Interactive effects occurred for TCS and UVR. Aquatic microbiota imbalance might have repercussions for the host/aquatic system, particularly in a realistic scenario/climate change perspective therefore, future ecotoxicological models should consider xenobiotics interactions with UVR.",

keywords = "Chemical stress, DGGE, Danio rerio embryos, Microbiota, Ultraviolet radiation",

author = "Oliveira, {Jacinta M.M.} and Almeida, {Ana Rita} and T{\^a}nia Pimentel and Andrade, {Thayres S.} and Henriques, {Jorge F.} and Soares, {Amadeu M.V.M.} and Susana Loureiro and Gomes, {Newton C.M.} and In{\^e}s Domingues",

note = "Funding Information: This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within projects CLIMATOX (PTDC/AAG-GLO/4059/2012) and through funding of CESAM ( CESAM/AMB/2013 ). Support was also given by the Post-Doc grant attributed to In{\^e}s Domingues ( SFRH/BPD/90521/2012 ) by FCT and by the Post-Doc grant attributed to Jacinta M. M. Oliveira (BPD/UI88/6463/2014), under the Research Project CENTRO-07-ST24-FEDER-002033 and Co-funded by QREN , Mais Centro - Programa Operacional Regional do Centro and European Union/European Regional Development Fund . Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd. All rights reserved.",

year = "2016",

month = jan,

day = "1",

doi = "10.1016/j.envpol.2015.10.039",

language = "English",

volume = "208",

pages = "626--636",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Effect of chemical stress and ultraviolet radiation in the bacterial communities of zebrafish embryos

AU - Oliveira, Jacinta M.M.

AU - Almeida, Ana Rita

AU - Pimentel, Tânia

AU - Andrade, Thayres S.

AU - Henriques, Jorge F.

AU - Soares, Amadeu M.V.M.

AU - Loureiro, Susana

AU - Gomes, Newton C.M.

AU - Domingues, Inês

N1 - Funding Information: This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within projects CLIMATOX (PTDC/AAG-GLO/4059/2012) and through funding of CESAM ( CESAM/AMB/2013 ). Support was also given by the Post-Doc grant attributed to Inês Domingues ( SFRH/BPD/90521/2012 ) by FCT and by the Post-Doc grant attributed to Jacinta M. M. Oliveira (BPD/UI88/6463/2014), under the Research Project CENTRO-07-ST24-FEDER-002033 and Co-funded by QREN , Mais Centro - Programa Operacional Regional do Centro and European Union/European Regional Development Fund . Publisher Copyright: © 2015 Elsevier Ltd. All rights reserved.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - This study aimed to assess the effect of ultraviolet radiation (UVR) and chemical stress (triclosan-TCS; potassium dichromate-PD; prochloraz-PCZ) on bacterial communities of zebrafish (Danio rerio) embryos (ZEBC). Embryos were exposed to two UVR intensities and two chemical concentrations not causing mortality or any developmental effect (equivalent to the No-Observed-Effect Concentration-NOEC; NOEC diluted by 10-NOEC/10). Effects on ZEBC were evaluated using denaturing gradient gel electrophoresis (DGGE) and interpreted considering structure, richness and diversity. ZEBC were affected by both stressors even at concentrations/doses not affecting the host-organism (survival/development). Yet, some stress-tolerant bacterial groups were revealed. The structure of the ZEBC was always affected, mainly due to xenobiotic presence. Richness and diversity decreased after exposure to NOEC of PD. Interactive effects occurred for TCS and UVR. Aquatic microbiota imbalance might have repercussions for the host/aquatic system, particularly in a realistic scenario/climate change perspective therefore, future ecotoxicological models should consider xenobiotics interactions with UVR.

AB - This study aimed to assess the effect of ultraviolet radiation (UVR) and chemical stress (triclosan-TCS; potassium dichromate-PD; prochloraz-PCZ) on bacterial communities of zebrafish (Danio rerio) embryos (ZEBC). Embryos were exposed to two UVR intensities and two chemical concentrations not causing mortality or any developmental effect (equivalent to the No-Observed-Effect Concentration-NOEC; NOEC diluted by 10-NOEC/10). Effects on ZEBC were evaluated using denaturing gradient gel electrophoresis (DGGE) and interpreted considering structure, richness and diversity. ZEBC were affected by both stressors even at concentrations/doses not affecting the host-organism (survival/development). Yet, some stress-tolerant bacterial groups were revealed. The structure of the ZEBC was always affected, mainly due to xenobiotic presence. Richness and diversity decreased after exposure to NOEC of PD. Interactive effects occurred for TCS and UVR. Aquatic microbiota imbalance might have repercussions for the host/aquatic system, particularly in a realistic scenario/climate change perspective therefore, future ecotoxicological models should consider xenobiotics interactions with UVR.

KW - Chemical stress

KW - DGGE

KW - Danio rerio embryos

KW - Microbiota

KW - Ultraviolet radiation

UR - http://www.scopus.com/inward/record.url?scp=84949489651&partnerID=8YFLogxK

U2 - 10.1016/j.envpol.2015.10.039

DO - 10.1016/j.envpol.2015.10.039

M3 - Article

C2 - 26552525

SN - 0269-7491

VL - 208

SP - 626

EP - 636

JO - Environmental Pollution

JF - Environmental Pollution

ER -

Effect of chemical stress and ultraviolet radiation in the bacterial communities of zebrafish embryos

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this