Effect of heat and thermosonication treatments on peroxidase inactivation kinetics in watercress (Nasturtium officinale)

Rui M. S. Cruz, Margarida C. Vieira, Cristina L. M. Silva*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

141 Citations (Scopus)

Abstract

The effect of heat and the combined heat/ultrasound (thermosonication) treatment on the inactivation kinetics of peroxidase in watercress (Nasturtium officinale) was studied in the temperature range of 40-92.5 °C. In the heat blanching processes, the enzyme kinetics showed a first-order biphasic inactivation model. The activation energies and the rates of the reaction at a reference temperature for both the heat-labile and heat-resistant fractions were, respectively, Ea1 = 421 ± 115 kJ mol-1 and Ea2 = 352 ± 81 kJ mol-1, k184.6°C= 18±14min-1 and k284.6°C=0.24±0.14min-1. The initial relative specific activity for both isoenzyme fractions were also estimated, being C 01 = 0.5 ± 0.08 μmol min-1 mg protein -1 and C02 = 0.5 ± 0.06 μmol min-1 mg protein-1, respectively. The application of thermosonication was studied to enable less severe thermal treatments and, therefore, improving the quality of the blanched product. In this treatment the enzyme kinetics showed a first-order model. The activation energy, the rate of reaction at a reference temperature and the initial relative specific activity were, respectively, Ea3 = 496 ± 65 kJ mol-1, k387.5°C= 10±2min-1 and C03 = 1 ± 0.05 μmol min-1 mg protein-1, proving that the enzyme became more heat labile. The present findings will help to design the blanching conditions for the production of a new and healthy frozen product, watercress (Nasturtium officinale), with minimized colour or flavour changes along its shelf life.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalJournal of Food Engineering
Volume72
Issue number1
DOIs
Publication statusPublished - 1 Jan 2006

Keywords

  • Heat
  • Inactivation
  • Kinetics
  • Modelling
  • Peroxidase
  • Ultrasound
  • Watercress (Nasturtium officinale)

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