Effect of storage conditions on stability of free and encapsulated in plain- or cysteine-supplemented alginate, Bifidobacterium animalis BB-12®

S. C. Sousa, E. A. Costa*, A. M. Gomes, M. M. Pintado, F. X. Malcata, J. P. Silva, J. M. Sousa Lobo, P. Costa, M. H. Amaral, M. F. Bahia, T. Rocha-Santos, D. Rodrigues, A. C. Freitas

*Corresponding author for this work

Research output: Contribution to conferenceAbstractpeer-review

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Abstract

The main objective of this research work was to study the viability of Bifidobacterium animalis BB-12® as free and calcium alginate-encapsulated cells, with or without cysteine, throughout storage, at four different temperatures. Extrusion by aerodynamically assisted flow was used to produce alginate and calcium alginate supplemented with L-cysteine·HCl microcapsules, containing B. animalis BB-12®. The microcapsules were suspended in Ringer solution in a 1:9 (g/mL) ratio, and stored at 21, 4, -20 and -80 ºC throughout six months, respectively. In parallel, the viability of free cells in cell suspension, was subjected to the same storage conditions and the corresponding viability assessed. Results showed that at 21, 4 and -20 ºC, the encapsulation did not have a protective effect—free cells maintained their viability throughout longer periods than encapsulated counterparts. At -80 ºC, encapsulation protected B. animalis BB-12® in comparison to the behavior of free cells. However, this effect was only observed in calcium alginate microcapsules supplemented with L-cysteine.HCl. After 180 days storage at -80 ºC, a 2 log cycle difference, in viable cells was observed between microcapsules with or without cysteine. The viable numbers of B. animalis BB-12® in microcapsules without cysteine was similar to that of free cells. In conclusion, alginate encapsulation revealed a protective effect on viability of B. animalis BB-12® stored at -80 ºC when supplemented with L-cysteine.HCl.
Original languageEnglish
Number of pages1
Publication statusPublished - Jul 2010
Event10th Biennial Congress of the Anaerobe Society of the Americas - Sheraton Society Hill Hotel, Philadelphia, United States
Duration: 7 Jul 201010 Jul 2010

Conference

Conference10th Biennial Congress of the Anaerobe Society of the Americas
Abbreviated titleAnaerobe 2010
Country/TerritoryUnited States
CityPhiladelphia
Period7/07/1010/07/10

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