Effect of various growth media upon survival during storage of freeze-dried Enterococcus faecalis and Enterococcus durans

A. S. Carvalho, J. Silva, P. Ho, P. Teixeira*, F. X. Malcata, P. Gibbs

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Aims: The effects of three different growth media (MRS, M17 and Lee's) on survival during freeze-drying and subsequent storage of six strains of Enterococcus faecalis and two strains of E. durans were investigated. Methods and Results: Distinct Enterococcus spp. strains were grown on M17, MRS and Lee's broth, freeze-dried and stored at 20°C in air under darkness. At regular intervals throughout storage, freeze-dried samples were rehydrated and then plated on M17 agar. Conclusions: A higher survival rate during storage of dried E. durans was obtained when growth occurred in MRS. The same effect was not observed, however, for the majority of E. faecalis strains, which clearly survived better in the dried state when this organism had been grown in M17 or Lee's medium. Significance and Impact of Study: The survival of the dried Enterococcus spp. tested during storage was shown to be strain-specific and dependent on the growth medium.

Original languageEnglish
Pages (from-to)947-952
Number of pages6
JournalJournal of Applied Microbiology
Volume94
Issue number6
DOIs
Publication statusPublished - 2003

Keywords

  • Enterococcus spp.
  • Growth medium
  • Preservation
  • Viability

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