Effects of acid mine drainage on the genetic diversity and structure of a natural population of Daphnia longispina

Nelson Martins; Catherine Bollinger; Ruth M. Harper; Rui Ribeiro

doi:10.1016/j.aquatox.2009.01.007

Effects of acid mine drainage on the genetic diversity and structure of a natural population of Daphnia longispina

Nelson Martins^*, Catherine Bollinger, Ruth M. Harper, Rui Ribeiro

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

The increase in resistance to contaminants can result in the loss of genetic diversity of impacted populations. In this work, the effects of acid mine drainage (AMD) on the genetic diversity and structure of a historically exposed population of Daphnia longispina were evaluated using amplified fragment length polymorphism (AFLP) analysis. Individual sensitivity to acute copper exposure was determined in order to characterize the populations in terms of metal tolerance and in an attempt to identify possible contaminant indicative bands (CIB). No reduction in genetic diversity was found in the AMD impacted site population, in comparison to two reference populations. However, the analysis of molecular variance indicated a significant genetic differentiation from the two reference populations and a significant correlation between individual genetic distance and tolerance. The different average tolerance of individuals presenting one specific AFLP band indicated the existence of one putative CIB.

Original language	English
Pages (from-to)	104-112
Number of pages	9
Journal	Aquatic Toxicology
Volume	92
Issue number	2
DOIs	https://doi.org/10.1016/j.aquatox.2009.01.007
Publication status	Published - 9 Apr 2009
Externally published	Yes

Keywords

AFLP
Daphnia
Metals
Microevolution
Population genetics

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10.1016/j.aquatox.2009.01.007

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title = "Effects of acid mine drainage on the genetic diversity and structure of a natural population of Daphnia longispina",

abstract = "The increase in resistance to contaminants can result in the loss of genetic diversity of impacted populations. In this work, the effects of acid mine drainage (AMD) on the genetic diversity and structure of a historically exposed population of Daphnia longispina were evaluated using amplified fragment length polymorphism (AFLP) analysis. Individual sensitivity to acute copper exposure was determined in order to characterize the populations in terms of metal tolerance and in an attempt to identify possible contaminant indicative bands (CIB). No reduction in genetic diversity was found in the AMD impacted site population, in comparison to two reference populations. However, the analysis of molecular variance indicated a significant genetic differentiation from the two reference populations and a significant correlation between individual genetic distance and tolerance. The different average tolerance of individuals presenting one specific AFLP band indicated the existence of one putative CIB.",

keywords = "AFLP, Daphnia, Metals, Microevolution, Population genetics",

author = "Nelson Martins and Catherine Bollinger and Harper, {Ruth M.} and Rui Ribeiro",

note = "Funding Information: N. Martins was a recipient from a Ph.D. grant from Funda{\c c}{\~a}o para a Ci{\^e}ncia e a Tecnologia (Portugal). The authors would also like to acknowledge I. Lopes and M. Moreira-Santos for the invaluable help in the field work and proofreading the article.",

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AU - Bollinger, Catherine

AU - Harper, Ruth M.

AU - Ribeiro, Rui

N1 - Funding Information: N. Martins was a recipient from a Ph.D. grant from Fundação para a Ciência e a Tecnologia (Portugal). The authors would also like to acknowledge I. Lopes and M. Moreira-Santos for the invaluable help in the field work and proofreading the article.

PY - 2009/4/9

Y1 - 2009/4/9

N2 - The increase in resistance to contaminants can result in the loss of genetic diversity of impacted populations. In this work, the effects of acid mine drainage (AMD) on the genetic diversity and structure of a historically exposed population of Daphnia longispina were evaluated using amplified fragment length polymorphism (AFLP) analysis. Individual sensitivity to acute copper exposure was determined in order to characterize the populations in terms of metal tolerance and in an attempt to identify possible contaminant indicative bands (CIB). No reduction in genetic diversity was found in the AMD impacted site population, in comparison to two reference populations. However, the analysis of molecular variance indicated a significant genetic differentiation from the two reference populations and a significant correlation between individual genetic distance and tolerance. The different average tolerance of individuals presenting one specific AFLP band indicated the existence of one putative CIB.

AB - The increase in resistance to contaminants can result in the loss of genetic diversity of impacted populations. In this work, the effects of acid mine drainage (AMD) on the genetic diversity and structure of a historically exposed population of Daphnia longispina were evaluated using amplified fragment length polymorphism (AFLP) analysis. Individual sensitivity to acute copper exposure was determined in order to characterize the populations in terms of metal tolerance and in an attempt to identify possible contaminant indicative bands (CIB). No reduction in genetic diversity was found in the AMD impacted site population, in comparison to two reference populations. However, the analysis of molecular variance indicated a significant genetic differentiation from the two reference populations and a significant correlation between individual genetic distance and tolerance. The different average tolerance of individuals presenting one specific AFLP band indicated the existence of one putative CIB.

KW - AFLP

KW - Daphnia

KW - Metals

KW - Microevolution

KW - Population genetics

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SN - 0166-445X

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JO - Aquatic Toxicology

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IS - 2

ER -

Effects of acid mine drainage on the genetic diversity and structure of a natural population of Daphnia longispina

Abstract

Keywords

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