TY - JOUR
T1 - Effects of UV radiation on the lipids and proteins of bacteria studied by mid-infrared spectroscopy
AU - Santos, Ana L.
AU - Moreirinha, Catarina
AU - Lopes, Diana
AU - Esteves, Ana Cristina
AU - Henriques, Isabel
AU - Almeida, Adelaide
AU - Domingues, M. Rosário M.
AU - Delgadillo, Ivonne
AU - Correia, António
AU - Cunha, Aîngela
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013/6/18
Y1 - 2013/6/18
N2 - Knowledge of the molecular effects of UV radiation (UVR) on bacteria can contribute to a better understanding of the environmental consequences of enhanced UV levels associated with global climate changes and will help to optimize UV-based disinfection strategies. In the present work, the effects of exposure to UVR in different spectral regions (UVC, 100-280 nm; UVB, 280-320 nm; and UVA, 320-400 nm) on the lipids and proteins of two bacterial strains (Acinetobacter sp. strain PT5I1.2G and Pseudomonas sp. strain NT5I1.2B) with distinct UV sensitivities were studied by mid-infrared spectroscopy. Exposure to UVR caused an increase in methyl groups associated with lipids, lipid oxidation, and also led to alterations in lipid composition, which were confirmed by gas chromatography. Additionally, mid-infrared spectroscopy revealed the effects of UVR on protein conformation and protein composition, which were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), oxidative damage to amino acids, and changes in the propionylation, glycosylation and/or phosphorylation status of cell proteins. Differences in the targets of UVR in the two strains tested were identified and may explain their discrepant UV sensitivities. The significance of the results is discussed from an ecological standpoint and with respect to potential improvements in UV-based disinfection technologies.
AB - Knowledge of the molecular effects of UV radiation (UVR) on bacteria can contribute to a better understanding of the environmental consequences of enhanced UV levels associated with global climate changes and will help to optimize UV-based disinfection strategies. In the present work, the effects of exposure to UVR in different spectral regions (UVC, 100-280 nm; UVB, 280-320 nm; and UVA, 320-400 nm) on the lipids and proteins of two bacterial strains (Acinetobacter sp. strain PT5I1.2G and Pseudomonas sp. strain NT5I1.2B) with distinct UV sensitivities were studied by mid-infrared spectroscopy. Exposure to UVR caused an increase in methyl groups associated with lipids, lipid oxidation, and also led to alterations in lipid composition, which were confirmed by gas chromatography. Additionally, mid-infrared spectroscopy revealed the effects of UVR on protein conformation and protein composition, which were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), oxidative damage to amino acids, and changes in the propionylation, glycosylation and/or phosphorylation status of cell proteins. Differences in the targets of UVR in the two strains tested were identified and may explain their discrepant UV sensitivities. The significance of the results is discussed from an ecological standpoint and with respect to potential improvements in UV-based disinfection technologies.
UR - http://www.scopus.com/inward/record.url?scp=84879197783&partnerID=8YFLogxK
U2 - 10.1021/es400660g
DO - 10.1021/es400660g
M3 - Article
C2 - 23692317
AN - SCOPUS:84879197783
SN - 0013-936X
VL - 47
SP - 6306
EP - 6315
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 12
ER -
