Elastins-based antimicrobial particles for delivery of bioactive compounds

Raul Machado*, André da Costa, Ana Margarida Pereira, José Carlos Rodriguez-Cabello, Margarida Casal

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


In the development of drug delivery systems, researchers pursue multifunctionality to target more complex problems, while maintaining biocompatibility and high encapsulation efficiency. Herein, we describe the preparation of noncytotoxic particles with intrinsic antimicrobial properties able to entrap bioactive compounds. The particles are composed of a recombinantly produced elastin-like recombinamer functionalized with an antimicrobial peptide, and are spontaneously formed in mild conditions by exploiting the thermoresponsiveness of the elastin-like portion. This chapter provides advice and methods for the preparation of the self-assembled antimicrobial particles, the evaluation of antimicrobial activity and cytotoxicity, and the basis to set up the methodology for the encapsulation of bioactive compounds.

Original languageEnglish
Title of host publicationNanoparticles in biology and medicine
Subtitle of host publicationmethods and protocols
EditorsEnrico Ferrari, Mikhail Soloviev
Place of PublicationNew York
PublisherHumana Press Inc.
Number of pages15
ISBN (Electronic)9781071603192
ISBN (Print)9781071603215, 9781071603185
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Antimicrobial peptide
  • Elastin-like recombinamer
  • Encapsulation
  • Particles
  • Self-assembly


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