@inbook{5ebfef85f4764d61923529d4032824c6,
title = "Elastins-based antimicrobial particles for delivery of bioactive compounds",
abstract = "In the development of drug delivery systems, researchers pursue multifunctionality to target more complex problems, while maintaining biocompatibility and high encapsulation efficiency. Herein, we describe the preparation of noncytotoxic particles with intrinsic antimicrobial properties able to entrap bioactive compounds. The particles are composed of a recombinantly produced elastin-like recombinamer functionalized with an antimicrobial peptide, and are spontaneously formed in mild conditions by exploiting the thermoresponsiveness of the elastin-like portion. This chapter provides advice and methods for the preparation of the self-assembled antimicrobial particles, the evaluation of antimicrobial activity and cytotoxicity, and the basis to set up the methodology for the encapsulation of bioactive compounds.",
keywords = "Antimicrobial peptide, Elastin-like recombinamer, Encapsulation, Particles, Self-assembly",
author = "Raul Machado and {da Costa}, Andr{\'e} and Pereira, {Ana Margarida} and Rodriguez-Cabello, {Jos{\'e} Carlos} and Margarida Casal",
note = "Funding Information: The manuscript was edited by Enrico Ferrari and Mikhail Soloviev. This work was supported by the strategic program UID/BIA/04050/2019 funded by national funds through FCT I.P. (Portugal). The present work was also supported by FCT I.P. within the ERANET IB-2 project FunBioPlas (grant number ERA-IB-15-089) with FCT reference ERAIB-2-6/0004/2014. This chapter is a result of the project EcoAgriFood [NORTE-01-0145-FEDER-000009], supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-0716-0319-2_3",
language = "English",
isbn = "9781071603215",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "29--43",
editor = "Enrico Ferrari and Mikhail Soloviev",
booktitle = "Nanoparticles in biology and medicine",
}