TY - JOUR
T1 - Enzymatic approach for the extraction of bioactive fractions from red, green and brown seaweeds
AU - Teixeira-Guedes, Catarina
AU - Gomes-Dias, Joana S.
AU - Cunha, Sara A.
AU - Pintado, Manuela E.
AU - Pereira, Ricardo N.
AU - Teixeira, José A.
AU - Rocha, Cristina M. R.
N1 - Funding Information:
This work was supported by the Portuguese Foundation for Science and Technology (FCT), under the scope of the strategic funding of UID/BIO/04469/2020 unit and LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020, by the project VALORMAR - Full valorization of marine resources: potential, technological innovation and new applications (call 10/SI/2016, reference 24517- Mobilizing R&TD Programs) co-funded by Compete 2020, Lisboa 2020, Algarve 2020, Portugal 2020 and European Union, through the European Regional Development Fund (ERDF) and by the project Bioeconorte (ref. NORTE-01-0145-FEDER-000070; co-funded by ERDF, through Norte2002). J. S. G.-D. and S.A.C. acknowledge the doctoral fellows 2021.06136. BD and SFRH/BD/144155/2019, respectively, provided by FCT. R. N. P. acknowledges FCT for its Assistant Research program under the scope of Scientific Stimulus Employment with reference CEECIND/02903/2017.
Funding Information:
This work was supported by the Portuguese Foundation for Science and Technology ( FCT ), under the scope of the strategic funding of UID/BIO/04469/2020 unit and LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020 , by the project VALORMAR - Full valorization of marine resources: potential, technological innovation and new applications (call 10/SI/2016, reference 24517- Mobilizing R&TD Programs) co-funded by Compete 2020, Lisboa 2020, Algarve 2020, Portugal 2020 and European Union , through the European Regional Development Fund ( ERDF ) and by the project Bioeconorte (ref. NORTE-01-0145-FEDER-000070 ; co-funded by ERDF, through Norte2002). J. S. G.-D. and S.A.C. acknowledge the doctoral fellows 2021.06136. BD and SFRH/BD/144155/2019 , respectively, provided by FCT. R. N. P. acknowledges FCT for its Assistant Research program under the scope of Scientific Stimulus Employment with reference CEECIND/02903/2017 .
Publisher Copyright:
© 2023 The Authors
PY - 2023/3
Y1 - 2023/3
N2 - Although several enzymatic approaches have been applied with this intent, the sequential use of enzymes covering both cellulolytic and proteolytic activity has never been performed in seaweeds. Sequential use of these enzymes improved the overall extraction yield by up to 160%, 30% and 80% in the different seaweeds when compared to the control condition (water extraction), use of a carbohydrases’ cocktail alone and use of proteases alone, respectively. Regarding the proximate composition of extracts, it proved to be an efficient approach for the solubilization of carbohydrates (up to 28% in G. vermiculophylla, 66% in P. dioica, 77% in U. rigida and 35% in F. vesiculosus) and protein (up to 55% in G. vermiculophylla, 47% in P. dioica, 52% in U. rigida and 42% in F. vesiculosus). For all biomasses, the combination of enzymes induced a significant increase in antioxidant activity, not only by the increase of phenolic compounds but also by the hydrolysis of protein to peptides. Moreover, extracts from red seaweeds displayed prebiotic activity which can be ascribed to their increased content in oligosaccharides and protein/peptides. Overall, the sequential use of enzymes with different activities demonstrated to be an efficient approach for extracting functional fractions to be used as functional ingredients to improve the nutritional value (e.g. in proteins) and/or to include antioxidant and prebiotic features in the food product.
AB - Although several enzymatic approaches have been applied with this intent, the sequential use of enzymes covering both cellulolytic and proteolytic activity has never been performed in seaweeds. Sequential use of these enzymes improved the overall extraction yield by up to 160%, 30% and 80% in the different seaweeds when compared to the control condition (water extraction), use of a carbohydrases’ cocktail alone and use of proteases alone, respectively. Regarding the proximate composition of extracts, it proved to be an efficient approach for the solubilization of carbohydrates (up to 28% in G. vermiculophylla, 66% in P. dioica, 77% in U. rigida and 35% in F. vesiculosus) and protein (up to 55% in G. vermiculophylla, 47% in P. dioica, 52% in U. rigida and 42% in F. vesiculosus). For all biomasses, the combination of enzymes induced a significant increase in antioxidant activity, not only by the increase of phenolic compounds but also by the hydrolysis of protein to peptides. Moreover, extracts from red seaweeds displayed prebiotic activity which can be ascribed to their increased content in oligosaccharides and protein/peptides. Overall, the sequential use of enzymes with different activities demonstrated to be an efficient approach for extracting functional fractions to be used as functional ingredients to improve the nutritional value (e.g. in proteins) and/or to include antioxidant and prebiotic features in the food product.
KW - Bioactive compounds carbohydrases
KW - Extraction
KW - Proteases
KW - Seaweeds
UR - http://www.scopus.com/inward/record.url?scp=85145972959&partnerID=8YFLogxK
U2 - 10.1016/j.fbp.2022.12.005
DO - 10.1016/j.fbp.2022.12.005
M3 - Article
SN - 0960-3085
VL - 138
SP - 25
EP - 39
JO - Food and Bioproducts Processing
JF - Food and Bioproducts Processing
ER -