TY - JOUR
T1 - Enzymatic determination of ethanol and glycerol by flow injection parallel multi-site detection
AU - Rangel, António O.S.S.
AU - Tóth, Ildikó V.
PY - 2000/7/10
Y1 - 2000/7/10
N2 - A flow injection method was developed for the sequential enzymatic determination of ethanol and glycerol in wines, using immobilised ethanol dehydrogenase and glycerol dehydrogenase, respectively. The enzymes were immobilised separately on alkylaminated controlled pore glass. A multi-site spectrophotometric detection system was used in parallel configuration to monitor the absorbance change in the two independent analytical channels. A 50-fold dilution of the samples was necessary before injection. The working range was between 0.05 and 0.5% (v/v) for the ethanol and between 0.03 and 0.3gl-1 for the glycerol determination, with corresponding detection limits of 2x10-3% (v/v) and 2x10-3gl-1. Relative standard deviations (R.S.D.) (n=9) lower than 2.3% for the ethanol and 2.1% for the glycerol determination were found. For 13 samples of different types of table and Port wines, the results showed good agreement with the corresponding reference procedures; a two level recovery study also showed good accuracy for the developed methods. The sampling rate was 10h-1, corresponding to 20 determinations per hour. Copyright (C) 2000 Elsevier Science B.V.
AB - A flow injection method was developed for the sequential enzymatic determination of ethanol and glycerol in wines, using immobilised ethanol dehydrogenase and glycerol dehydrogenase, respectively. The enzymes were immobilised separately on alkylaminated controlled pore glass. A multi-site spectrophotometric detection system was used in parallel configuration to monitor the absorbance change in the two independent analytical channels. A 50-fold dilution of the samples was necessary before injection. The working range was between 0.05 and 0.5% (v/v) for the ethanol and between 0.03 and 0.3gl-1 for the glycerol determination, with corresponding detection limits of 2x10-3% (v/v) and 2x10-3gl-1. Relative standard deviations (R.S.D.) (n=9) lower than 2.3% for the ethanol and 2.1% for the glycerol determination were found. For 13 samples of different types of table and Port wines, the results showed good agreement with the corresponding reference procedures; a two level recovery study also showed good accuracy for the developed methods. The sampling rate was 10h-1, corresponding to 20 determinations per hour. Copyright (C) 2000 Elsevier Science B.V.
KW - Ethanol
KW - Glycerol
KW - Multi-site detection
KW - Sequential flow injection
KW - Spectrophotometric enzymatic analysis
KW - Wine
UR - http://www.scopus.com/inward/record.url?scp=17744416318&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(00)00905-3
DO - 10.1016/S0003-2670(00)00905-3
M3 - Article
AN - SCOPUS:17744416318
SN - 0003-2670
VL - 416
SP - 205
EP - 210
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -