Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia

Regine Labeque; Claudy J.P. Mullon; João Paulo M. Ferreira; Robert S. Lees; Robert Langer

doi:10.1073/pnas.90.8.3476

Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia

Regine Labeque, Claudy J.P. Mullon, João Paulo M. Ferreira, Robert S. Lees, Robert Langer^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Phospholipase A₂ (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A₂-modifted human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A₂. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.

Original language	English
Pages (from-to)	3476-3480
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	90
Issue number	8
DOIs	https://doi.org/10.1073/pnas.90.8.3476
Publication status	Published - 15 Apr 1993
Externally published	Yes

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title = "Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia",

abstract = "Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modifted human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A2. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.",

author = "Regine Labeque and Mullon, {Claudy J.P.} and Ferreira, {Jo{\~a}o Paulo M.} and Lees, {Robert S.} and Robert Langer",

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Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia. / Labeque, Regine; Mullon, Claudy J.P.; Ferreira, João Paulo M. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 8, 15.04.1993, p. 3476-3480.

Research output: Contribution to journal › Article › peer-review

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T1 - Enzymatic modification of plasma low density lipoproteins in rabbits

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AU - Labeque, Regine

AU - Mullon, Claudy J.P.

AU - Ferreira, João Paulo M.

AU - Lees, Robert S.

AU - Langer, Robert

PY - 1993/4/15

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AB - Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modifted human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A2. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.

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Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia

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