Epifluorescence microscope methods for bacterial enumeration in a 4-chlorophenol degrading consortium

C. C. Pacheco, C. C. Alves, L. Barreiros, P. M.L. Castro*, P. C.M. Teixeira

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)
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Abstract

Epifluorescence microscope methods, namely BacLight, direct epifluorescence filter technique and Rhodamine 123, consistently underestimated plate bacterial counts in a 4-chlorophenol degrading consortium. Cells capable of passing through 0.2 μm filters, referred as 'ultramicrocells', were found. Although cell counts were higher when traditional methods were used, BacLight and direct epifluorescence filter technique were convenient techniques for the systematic monitoring of bacteria involved in biodegradation processes, as results were consistent and available within a short time.

Original languageEnglish
Pages (from-to)2089-2092
Number of pages4
JournalBiotechnology Letters
Volume25
Issue number24
DOIs
Publication statusPublished - Dec 2003

Keywords

  • Degrading consortia
  • Fluorescence dyes
  • Starvation
  • Ultramicrocells
  • Viable but non-culturable bacteria

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