Estrogen-deficiency effect on the composition of dental enamel: a pilot study

Gustavo V. de Oliveira Fernandes; Erika C. Küchler; Marjorie A. Omori; Guido A. Marañón-Vasquez; Lucas R. Teixeira; Jorge E. Léon; Celia M. C. de França Lopes; Flares Baratto-Filho; Paulo Nelson-Filho; Raquel F. Gerlach; Isabela R. Madalena

doi:10.4103/SDJ.SDJ_61_20

Estrogen-deficiency effect on the composition of dental enamel: a pilot study

Gustavo V. de Oliveira Fernandes, Erika C. Küchler, Marjorie A. Omori, Guido A. Marañón-Vasquez, Lucas R. Teixeira, Jorge E. Léon, Celia M. C. de França Lopes, Flares Baratto-Filho, Paulo Nelson-Filho, Raquel F. Gerlach, Isabela R. Madalena

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Abstract

Background: Tooth enamel mineralization is assumed to be a target of endogenous estrogen imbalances. Objective: To evaluate the effect of estrogen deficiency during amelogenesis on the mineral composition of dental enamel. Methods: Ten female Wistar Hannover rats were randomly divided into two groups according to the intervention received: ovariectomy surgery (OVX, experimental) and fictitious surgery (SHAM, control). After 21 days, the rats of both groups were euthanized, and the upper incisors were extracted for analysis of the mineral composition by energy-dispersive X-ray fluorescence. The sensitivity of the enamel organ to estrogen was evaluated in both groups by immunohistochemical analysis of the odontogenic region of the lower incisors for the presence of estrogen receptors alpha and beta (ERα and ERβ, respectively) in ameloblasts in the maturation stage. Differences in the mineral composition between groups were compared using Student’s t-test (P < 0.05). Results: No statistically significant difference was detected in the mineral composition between the OVX and SHAM groups (P > 0.05). ERα was immunostained in the ameloblasts of both groups. Conclusion: Although ameloblasts express ERα, estrogen deficiency during amelogenesis did not appear to affect the dental enamel composition in this murine model.

Original language	English
Pages (from-to)	57-62
Number of pages	6
Journal	Scientific Dental Journal
Volume	5
Issue number	2
DOIs	https://doi.org/10.4103/SDJ.SDJ_61_20
Publication status	Published - 23 Jun 2021

Keywords

Amelogenesis
Dental enamel
Estrogens

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10.4103/SDJ.SDJ_61_20Licence: CC BY-NC-SA

32028856Final published version, 1.89 MBLicence: CC BY-NC-SA

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CIIS: Center for Interdisciplinary Research in Health
Barros, M. (PI), Rosa, N. (Researcher), Correia, M. J. (Researcher), Caldas, A. C. (Researcher), Amado, J. C. (Researcher), Figueiredo, A. S. (Researcher), Esteves, A. C. (Researcher), Mineiro, A. (Researcher), Abreu, A. M. (Researcher), Duarte, A. S. (Researcher), Almeida, S. F. (Researcher), Correia, A. (Researcher), Moura, A. (Researcher), Almeida, A. (Researcher), Araújo, B. (Researcher), Moura-Netto, C. (Researcher), Ferrito, C. (Researcher), Pais-Vieira, C. (Researcher), Festas, C. (Researcher), Marques-Vieira, C. (Researcher), Catré, D. (Researcher), Nunes, E. (Researcher), Jesus, É. (Researcher), Ribeiro, F. (Researcher), Rosário, F. (Researcher), Fernandes, G. (Researcher), Rato, J. R. (Scholarship holder), Salgado, J. R. (Researcher), Neves-Amado, J. (Researcher), Amendoeira, J. (Researcher), Sá, L. (Researcher), Capelas, M. L. (Researcher), Vieira, M. M. (Researcher), Nunes, M. V. S. (Researcher), Cardoso, M. (Researcher), Veiga, N. J. (Researcher), Fonseca, P. (Researcher), Correia, P. N. (Researcher), Couto, P. (Researcher), Pontífice-Sousa, P. (Researcher), Ravasco, P. (Researcher), Carvalho, P. V. D. (Researcher), Alves, P. (Researcher), Melo, P. (Researcher), Silva, R. (Researcher), Canaipa, R. (Researcher), Noites, R. (Researcher), Rio, R. (Researcher), Almeida, S. (Researcher), Deodato, S. (Researcher), Caldeira, S. (Researcher), Silva, S. (Researcher), Borges, T. (Researcher), Silva, V. (Researcher), Charepe, Z. (Researcher), Rodrigues-Pires, F. (Volunteer), Veludo, F. (Researcher), Carmo, H. (Scholarship holder), Romeiro, J. (Student), Melo, M. (Researcher), Braga, M. (Researcher), Amaral, T. (Researcher), Moreira, M. A. (Researcher), Guerra, N. (Student), Santos, P. (Researcher), Paço, S. (Student), Lynce, S. (Scholarship holder), Miguel, S. (Student), Costa, T. (Researcher), Silva-Neves, V. (Researcher), Silva, A. (Researcher), Carvalho, A. R. (Researcher), Almeida, B. (Researcher), Figueiredo, C. (Researcher), Esteves, E. (Scholarship holder), Araújo, F. M. (Researcher), Garcia, J. G. (Researcher), Santos, L. (Researcher), Santos, N. M. D. (Researcher), Lopes, P. (Researcher), Bornes, R. (Researcher), Silva, R. (Researcher), Costa, S. (Researcher), Silva, S. M. (Researcher), Marques, T. (Researcher), Almeida, A. (Researcher), Santos, M. (Scholarship holder), Santos, P. (Researcher), Miguel, S. (Researcher), Mendes, K. (Researcher), Gomes, A. P. (Researcher), Henriques, J. M. P. (Researcher), Costa, H. A. F. P. (Researcher) & Ribeiro, P. (Researcher)
Fundação para a Ciência e a Tecnologia
1/01/20 → 31/12/24
Project: Research

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Fernandes, G. V. D. O., Küchler, E. C., Omori, M. A., Marañón-Vasquez, G. A., Teixeira, L. R., Léon, J. E., Lopes, C. M. C. D. F., Baratto-Filho, F., Nelson-Filho, P., Gerlach, R. F., & Madalena, I. R. (2021). Estrogen-deficiency effect on the composition of dental enamel: a pilot study. Scientific Dental Journal, 5(2), 57-62. https://doi.org/10.4103/SDJ.SDJ_61_20

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abstract = "Background: Tooth enamel mineralization is assumed to be a target of endogenous estrogen imbalances. Objective: To evaluate the effect of estrogen deficiency during amelogenesis on the mineral composition of dental enamel. Methods: Ten female Wistar Hannover rats were randomly divided into two groups according to the intervention received: ovariectomy surgery (OVX, experimental) and fictitious surgery (SHAM, control). After 21 days, the rats of both groups were euthanized, and the upper incisors were extracted for analysis of the mineral composition by energy-dispersive X-ray fluorescence. The sensitivity of the enamel organ to estrogen was evaluated in both groups by immunohistochemical analysis of the odontogenic region of the lower incisors for the presence of estrogen receptors alpha and beta (ERα and ERβ, respectively) in ameloblasts in the maturation stage. Differences in the mineral composition between groups were compared using Student{\textquoteright}s t-test (P < 0.05). Results: No statistically significant difference was detected in the mineral composition between the OVX and SHAM groups (P > 0.05). ERα was immunostained in the ameloblasts of both groups. Conclusion: Although ameloblasts express ERα, estrogen deficiency during amelogenesis did not appear to affect the dental enamel composition in this murine model.",

keywords = "Amelogenesis, Dental enamel, Estrogens",

author = "Fernandes, {Gustavo V. de Oliveira} and K{\"u}chler, {Erika C.} and Omori, {Marjorie A.} and Mara{\~n}{\'o}n-Vasquez, {Guido A.} and Teixeira, {Lucas R.} and L{\'e}on, {Jorge E.} and Lopes, {Celia M. C. de Fran{\c c}a} and Flares Baratto-Filho and Paulo Nelson-Filho and Gerlach, {Raquel F.} and Madalena, {Isabela R.}",

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month = jun,

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doi = "10.4103/SDJ.SDJ_61_20",

language = "English",

volume = "5",

pages = "57--62",

journal = "Scientific Dental Journal",

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TY - JOUR

T1 - Estrogen-deficiency effect on the composition of dental enamel

T2 - a pilot study

AU - Fernandes, Gustavo V. de Oliveira

AU - Küchler, Erika C.

AU - Omori, Marjorie A.

AU - Marañón-Vasquez, Guido A.

AU - Teixeira, Lucas R.

AU - Léon, Jorge E.

AU - Lopes, Celia M. C. de França

AU - Baratto-Filho, Flares

AU - Nelson-Filho, Paulo

AU - Gerlach, Raquel F.

AU - Madalena, Isabela R.

PY - 2021/6/23

Y1 - 2021/6/23

N2 - Background: Tooth enamel mineralization is assumed to be a target of endogenous estrogen imbalances. Objective: To evaluate the effect of estrogen deficiency during amelogenesis on the mineral composition of dental enamel. Methods: Ten female Wistar Hannover rats were randomly divided into two groups according to the intervention received: ovariectomy surgery (OVX, experimental) and fictitious surgery (SHAM, control). After 21 days, the rats of both groups were euthanized, and the upper incisors were extracted for analysis of the mineral composition by energy-dispersive X-ray fluorescence. The sensitivity of the enamel organ to estrogen was evaluated in both groups by immunohistochemical analysis of the odontogenic region of the lower incisors for the presence of estrogen receptors alpha and beta (ERα and ERβ, respectively) in ameloblasts in the maturation stage. Differences in the mineral composition between groups were compared using Student’s t-test (P < 0.05). Results: No statistically significant difference was detected in the mineral composition between the OVX and SHAM groups (P > 0.05). ERα was immunostained in the ameloblasts of both groups. Conclusion: Although ameloblasts express ERα, estrogen deficiency during amelogenesis did not appear to affect the dental enamel composition in this murine model.

AB - Background: Tooth enamel mineralization is assumed to be a target of endogenous estrogen imbalances. Objective: To evaluate the effect of estrogen deficiency during amelogenesis on the mineral composition of dental enamel. Methods: Ten female Wistar Hannover rats were randomly divided into two groups according to the intervention received: ovariectomy surgery (OVX, experimental) and fictitious surgery (SHAM, control). After 21 days, the rats of both groups were euthanized, and the upper incisors were extracted for analysis of the mineral composition by energy-dispersive X-ray fluorescence. The sensitivity of the enamel organ to estrogen was evaluated in both groups by immunohistochemical analysis of the odontogenic region of the lower incisors for the presence of estrogen receptors alpha and beta (ERα and ERβ, respectively) in ameloblasts in the maturation stage. Differences in the mineral composition between groups were compared using Student’s t-test (P < 0.05). Results: No statistically significant difference was detected in the mineral composition between the OVX and SHAM groups (P > 0.05). ERα was immunostained in the ameloblasts of both groups. Conclusion: Although ameloblasts express ERα, estrogen deficiency during amelogenesis did not appear to affect the dental enamel composition in this murine model.

KW - Amelogenesis

KW - Dental enamel

KW - Estrogens

U2 - 10.4103/SDJ.SDJ_61_20

DO - 10.4103/SDJ.SDJ_61_20

M3 - Article

SN - 2541-321X

VL - 5

SP - 57

EP - 62

JO - Scientific Dental Journal

JF - Scientific Dental Journal

IS - 2

ER -

Estrogen-deficiency effect on the composition of dental enamel: a pilot study

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Keywords

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