TY - JOUR
T1 - Eucalyptus globulus bark as a source of polyphenolic compounds with biological activity
AU - Mota, Maria Inês Ferreira da
AU - Pinto, Paula Cristina de Oliveira Rodrigues
AU - Novo, Catarina Cardoso
AU - Sousa, Gabriel Duarte Almeida
AU - Guerreiro, Olinda Rosa Fragoso da Neves
AU - Guerra, Ânegela da Conceição Relvas
AU - Duarte, Maria Fátima Pereira
AU - Rodrigues, Alírio Egídio
PY - 2013/1
Y1 - 2013/1
N2 - Eucalyptus globulus bark, one of the main by-products of the pulp and paper industry in Southern Europe, is a potential source of valuable chemicals. In this work, chemical composition of E. globulus bark was studied with detail, including its carbohydrate composition. Response surface methodology (RSM) modeling and optimization was developed for the selective extraction of polyphenolic material from E. globulus bark. The RSM method was based on the Box-Behnken design, aiming to obtain the optimal combination of extraction conditions considering the parameters ethanol percentage in the extraction medium, temperature and time. Conditions for maximum of polyphenols in the extract are 52% ethanol, extraction temperature of 82.5°C and extraction time of 264 min. The polyphenolic compounds - quantified as gallic acid equivalents - in the extract produced at the optimal conditions was 32%, corresponding to about 2% of bark weight, with a carbohydrate co-extraction of about 1.6% of bark carbohydrate content. Some of the extracts revealed low values of IC50 against human breast cancer cells, indicative of high biological activity. This work has demonstrated the potential of E. globulus bark as a source of polyphenolic compounds with anti-proliferative activity and gives a positive contribution to the increase in products portfolio diversification in pulp industry and biorefineries.
AB - Eucalyptus globulus bark, one of the main by-products of the pulp and paper industry in Southern Europe, is a potential source of valuable chemicals. In this work, chemical composition of E. globulus bark was studied with detail, including its carbohydrate composition. Response surface methodology (RSM) modeling and optimization was developed for the selective extraction of polyphenolic material from E. globulus bark. The RSM method was based on the Box-Behnken design, aiming to obtain the optimal combination of extraction conditions considering the parameters ethanol percentage in the extraction medium, temperature and time. Conditions for maximum of polyphenols in the extract are 52% ethanol, extraction temperature of 82.5°C and extraction time of 264 min. The polyphenolic compounds - quantified as gallic acid equivalents - in the extract produced at the optimal conditions was 32%, corresponding to about 2% of bark weight, with a carbohydrate co-extraction of about 1.6% of bark carbohydrate content. Some of the extracts revealed low values of IC50 against human breast cancer cells, indicative of high biological activity. This work has demonstrated the potential of E. globulus bark as a source of polyphenolic compounds with anti-proliferative activity and gives a positive contribution to the increase in products portfolio diversification in pulp industry and biorefineries.
KW - Bark
KW - Bioactivity
KW - Carbohydrates
KW - Extraction optimization
KW - Phenolic compounds
UR - https://www.scopus.com/pages/publications/84880122308
M3 - Article
SN - 0031-1057
VL - 74
SP - 57
EP - 64
JO - O Papel (Brazil)
JF - O Papel (Brazil)
IS - 1
ER -
