TY - JOUR
T1 - Evaluation of chitoligosaccharides effect upon probiotic bacteria
AU - Fernandes, João C.
AU - Eaton, Peter
AU - Franco, Isabel
AU - Ramos, Óscar S.
AU - Sousa, Sérgio
AU - Nascimento, Henrique
AU - Gomes, Ana
AU - Santos-Silva, Alice
AU - Malcata, F. Xavier
AU - Pintado, Manuela E.
PY - 2012/1/1
Y1 - 2012/1/1
N2 - The main objective of the present study was to evaluate the antibacterial effect - through the determination of minimum inhibitory (and lethal) concentrations, as well as the possible prebiotic potential of chitooligosaccharides (COS) - through the determination of growth curves, on Bifidobacterium animalis Bb12, Bifidobacterium animalis Bo and Lactobacillus acidophilus Ki. Atomic force microscopy was further used to obtain high resolution images of COS effects upon the cell morphology. Our results demonstrate that COS do not stimulate the growth of those strains, neither the strains are capable of using COS as a primary source of carbon. Analysis of morphology when exposed to inhibitory/bactericidal concentrations, suggested that COS do not exert any direct damage upon the bacteria structure, instead the bacteria are apparently covered by COS, which likely prevent nutrient uptake.
AB - The main objective of the present study was to evaluate the antibacterial effect - through the determination of minimum inhibitory (and lethal) concentrations, as well as the possible prebiotic potential of chitooligosaccharides (COS) - through the determination of growth curves, on Bifidobacterium animalis Bb12, Bifidobacterium animalis Bo and Lactobacillus acidophilus Ki. Atomic force microscopy was further used to obtain high resolution images of COS effects upon the cell morphology. Our results demonstrate that COS do not stimulate the growth of those strains, neither the strains are capable of using COS as a primary source of carbon. Analysis of morphology when exposed to inhibitory/bactericidal concentrations, suggested that COS do not exert any direct damage upon the bacteria structure, instead the bacteria are apparently covered by COS, which likely prevent nutrient uptake.
KW - Atomic force microscopy
KW - Chitooligosaccharides
KW - Chitosan
KW - Probiotic bacteria
UR - http://www.scopus.com/inward/record.url?scp=83555165002&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2011.10.011
DO - 10.1016/j.ijbiomac.2011.10.011
M3 - Article
C2 - 22024313
AN - SCOPUS:83555165002
SN - 0141-8130
VL - 50
SP - 148
EP - 152
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
IS - 1
ER -