TY - JOUR
T1 - Evolution of the NET (NocA, Nlz, Elbow, TLP-1) protein family in metazoans
T2 - insights from expression data and phylogenetic analysis
AU - Pereira, Filipe
AU - Duarte-Pereira, Sara
AU - Silva, Raquel M.
AU - Da Costa, Luís Teixeira
AU - Pereira-Castro, Isabel
N1 - Publisher Copyright:
© 2016 The Author(s).
Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 2016/12/8
Y1 - 2016/12/8
N2 - The NET (for NocA, Nlz, Elbow, TLP-1) protein family is a group of conserved zinc finger proteins linked to embryonic development and recently associated with breast cancer. The members of this family act as transcriptional repressors interacting with both class I histone deacetylases and Groucho/TLE co-repressors. In Drosophila, the NET family members Elbow and NocA are vital for the development of tracheae, eyes, wings and legs, whereas in vertebrates ZNF703 and ZNF503 are important for the development of the nervous system, eyes and limbs. Despite the relevance of this protein family in embryogenesis and cancer, many aspects of its origin and evolution remain unknown. Here, we show that NET family members are present and expressed in multiple metazoan lineages, from cnidarians to vertebrates. We identified several protein domains conserved in all metazoan species or in specific taxonomic groups. Our phylogenetic analysis suggests that the NET family emerged in the last common ancestor of cnidarians and bilaterians and that several rounds of independent events of gene duplication occurred throughout evolution. Overall, we provide novel data on the expression and evolutionary history of the NET family that can be relevant to understanding its biological role in both normal conditions and disease.
AB - The NET (for NocA, Nlz, Elbow, TLP-1) protein family is a group of conserved zinc finger proteins linked to embryonic development and recently associated with breast cancer. The members of this family act as transcriptional repressors interacting with both class I histone deacetylases and Groucho/TLE co-repressors. In Drosophila, the NET family members Elbow and NocA are vital for the development of tracheae, eyes, wings and legs, whereas in vertebrates ZNF703 and ZNF503 are important for the development of the nervous system, eyes and limbs. Despite the relevance of this protein family in embryogenesis and cancer, many aspects of its origin and evolution remain unknown. Here, we show that NET family members are present and expressed in multiple metazoan lineages, from cnidarians to vertebrates. We identified several protein domains conserved in all metazoan species or in specific taxonomic groups. Our phylogenetic analysis suggests that the NET family emerged in the last common ancestor of cnidarians and bilaterians and that several rounds of independent events of gene duplication occurred throughout evolution. Overall, we provide novel data on the expression and evolutionary history of the NET family that can be relevant to understanding its biological role in both normal conditions and disease.
UR - http://www.scopus.com/inward/record.url?scp=85003583805&partnerID=8YFLogxK
U2 - 10.1038/srep38383
DO - 10.1038/srep38383
M3 - Article
C2 - 27929068
AN - SCOPUS:85003583805
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 38383
ER -