Expanding the potential of self-assembled silk fibroin as aerogel particles for tissue regeneration

Beatriz G. Bernardes, Sara Baptista-Silva, Carlos Illanes-Bordomás, Rui Magalhães, Juliana Rosa Dias, Nuno M. F. Alves, Raquel Costa*, Carlos A. García-González*, Ana Leite Oliveira*

*Corresponding author for this work

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Abstract

A newly produced silk fibroin (SF) aerogel particulate system using a supercritical carbon dioxide (scCO2)-assisted drying technology is herein proposed for biomedical applications. Different concentrations of silk fibroin (3%, 5%, and 7% (w/v)) were explored to investigate the potential of this technology to produce size- and porosity-controlled particles. Laser diffraction, helium pycnometry, nitrogen adsorption–desorption analysis and Fourier Transform Infrared with Attenuated Total Reflectance (FTIR-ATR) spectroscopy were performed to characterize the physicochemical properties of the material. The enzymatic degradation profile of the SF aerogel particles was evaluated by immersion in protease XIV solution, and the biological properties by cell viability and cell proliferation assays. The obtained aerogel particles were mesoporous with high and concentration dependent specific surface area (203–326 m2/g). They displayed significant antioxidant activity and sustained degradation in the presence of protease XIV enzyme. The in vitro assessment using human dermal fibroblasts (HDF) confirm the particles’ biocompatibility, as well as the enhancement in cell viability and proliferation.
Original languageEnglish
Article number2605
Number of pages19
JournalPharmaceutics
Volume15
Issue number11
DOIs
Publication statusPublished - 9 Nov 2023

Keywords

  • Aerogel
  • Particles and silk fibroin
  • Tissue engineering

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