Abstract
Solid lipid nanoparticles (SLNs) produced from multiple emulsions technology theoretically enclose an inner aqueous compartment suitable for hydrophilic biomolecules. This paper reports a 33 full factorial design study to optimize SLNs formulations for hydrophilic biomolecules. The concentrations of solid lipid, lipophilic and hydrophilic emulsifiers were set as the 3 independent variables. Mean particle size (Z-Ave), polydispersity index (PI) and zeta potential (ZP) were set as the dependent variables. The selected optimized parameters were set as 1.0wt% of solid lipid, 0.25wt% of lipophilic emulsifier and 1.5wt% of hydrophilic emulsifier. The coating of SLNs with sodium alginate was found to improve the ZP of the lipid particles and these results suggest that the ideal concentration was 0.75wt%. The influence of low pH (i.e., about 2-3) in the inner aqueous phase was stronger than higher pH values, contributing for the production of larger droplet sizes. Nevertheless, these systems can be useful for the incorporation of biomolecules requiring a pH ranging between 4 and 10. SLNs based on multiple emulsions technology were found to be a promising approach for the incorporation of several hydrophilic drugs, such as proteins and peptides.
Original language | English |
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Pages (from-to) | 84-89 |
Number of pages | 6 |
Journal | Colloids and Surfaces B: Biointerfaces |
Volume | 100 |
DOIs | |
Publication status | Published - 1 Dec 2012 |
Externally published | Yes |
Keywords
- Factorial design
- Hydrophilic drugs
- Lipid nanocarriers
- Multiple emulsion
- Solid lipid nanoparticles