Experimental factorial design applied to mucoadhesive lipid nanoparticles via multiple emulsion process

Joana F. Fangueiro, Tatiana Andreani, Maria A. Egea, Maria L. Garcia, Selma B. Souto, Eliana B. Souto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

Solid lipid nanoparticles (SLNs) produced from multiple emulsions technology theoretically enclose an inner aqueous compartment suitable for hydrophilic biomolecules. This paper reports a 33 full factorial design study to optimize SLNs formulations for hydrophilic biomolecules. The concentrations of solid lipid, lipophilic and hydrophilic emulsifiers were set as the 3 independent variables. Mean particle size (Z-Ave), polydispersity index (PI) and zeta potential (ZP) were set as the dependent variables. The selected optimized parameters were set as 1.0wt% of solid lipid, 0.25wt% of lipophilic emulsifier and 1.5wt% of hydrophilic emulsifier. The coating of SLNs with sodium alginate was found to improve the ZP of the lipid particles and these results suggest that the ideal concentration was 0.75wt%. The influence of low pH (i.e., about 2-3) in the inner aqueous phase was stronger than higher pH values, contributing for the production of larger droplet sizes. Nevertheless, these systems can be useful for the incorporation of biomolecules requiring a pH ranging between 4 and 10. SLNs based on multiple emulsions technology were found to be a promising approach for the incorporation of several hydrophilic drugs, such as proteins and peptides.

Original languageEnglish
Pages (from-to)84-89
Number of pages6
JournalColloids and Surfaces B: Biointerfaces
Volume100
DOIs
Publication statusPublished - 1 Dec 2012
Externally publishedYes

Keywords

  • Factorial design
  • Hydrophilic drugs
  • Lipid nanocarriers
  • Multiple emulsion
  • Solid lipid nanoparticles

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