Exploiting the bead injection LOV approach to carry out spectrophotometric assays in wine: application to the determination of iron

Susana S. M. P. Vidigal; Ildikó V. Tóth; António O. S. S. Rangel

doi:10.1016/j.talanta.2011.01.041

Exploiting the bead injection LOV approach to carry out spectrophotometric assays in wine: application to the determination of iron

Susana S. M. P. Vidigal, Ildikó V. Tóth, António O. S. S. Rangel^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

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Abstract

A sequential injection lab-on-valve (SI-LOV) system was used to develop a new methodology for the determination of iron in wine samples exploiting the bead injection (BI) concept for solid phase extraction and spectrophotometric measurement. Nitrilotriacetic Acid (NTA) Superflow resin was used to build the bead column of the flow through sensor. The iron (III) ions were retained by the bead column and react with SCN- producing an intense red colour. The change in absorbance was monitored spectrophotometrically on the optosensor at 480 nm. It was possible to achieve a linear range of 0.09-5.0 mg L-1 of iron, with low sample and reagent consumption; 500 μL of sample, 15 μmol of SCN-, and 9 μmol of H2O2, per assay. The proposed method was successfully applied to the determination of iron in wine, with no previous treatment other than dilution, and to other food samples.

Original language	English
Pages (from-to)	1298-1303
Number of pages	6
Journal	Talanta
Volume	84
Issue number	5
DOIs	https://doi.org/10.1016/j.talanta.2011.01.041
Publication status	Published - 15 Jun 2011
Event	16th International Conference on Flow Injection Analysis - Garden Sea View Resort Hotel, Pattaya, Thailand Duration: 25 Apr 2010 → 30 Apr 2010 Conference number: 16

Keywords

Bead injection
Iron
Sequential injection lab-on-valve
Solid phase spectrometry
Wine

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10.1016/j.talanta.2011.01.041

http://hdl.handle.net/10400.14/7430

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title = "Exploiting the bead injection LOV approach to carry out spectrophotometric assays in wine: application to the determination of iron",

abstract = "A sequential injection lab-on-valve (SI-LOV) system was used to develop a new methodology for the determination of iron in wine samples exploiting the bead injection (BI) concept for solid phase extraction and spectrophotometric measurement. Nitrilotriacetic Acid (NTA) Superflow resin was used to build the bead column of the flow through sensor. The iron (III) ions were retained by the bead column and react with SCN- producing an intense red colour. The change in absorbance was monitored spectrophotometrically on the optosensor at 480 nm. It was possible to achieve a linear range of 0.09-5.0 mg L-1 of iron, with low sample and reagent consumption; 500 μL of sample, 15 μmol of SCN-, and 9 μmol of H2O2, per assay. The proposed method was successfully applied to the determination of iron in wine, with no previous treatment other than dilution, and to other food samples.",

keywords = "Bead injection, Iron, Sequential injection lab-on-valve, Solid phase spectrometry, Wine",

author = "Vidigal, {Susana S. M. P.} and T{\'o}th, {Ildik{\'o} V.} and Rangel, {Ant{\'o}nio O. S. S.}",

year = "2011",

month = jun,

day = "15",

doi = "10.1016/j.talanta.2011.01.041",

language = "English",

volume = "84",

pages = "1298--1303",

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T2 - 16th International Conference on Flow Injection Analysis

AU - Vidigal, Susana S. M. P.

AU - Tóth, Ildikó V.

AU - Rangel, António O. S. S.

N1 - Conference code: 16

PY - 2011/6/15

Y1 - 2011/6/15

N2 - A sequential injection lab-on-valve (SI-LOV) system was used to develop a new methodology for the determination of iron in wine samples exploiting the bead injection (BI) concept for solid phase extraction and spectrophotometric measurement. Nitrilotriacetic Acid (NTA) Superflow resin was used to build the bead column of the flow through sensor. The iron (III) ions were retained by the bead column and react with SCN- producing an intense red colour. The change in absorbance was monitored spectrophotometrically on the optosensor at 480 nm. It was possible to achieve a linear range of 0.09-5.0 mg L-1 of iron, with low sample and reagent consumption; 500 μL of sample, 15 μmol of SCN-, and 9 μmol of H2O2, per assay. The proposed method was successfully applied to the determination of iron in wine, with no previous treatment other than dilution, and to other food samples.

AB - A sequential injection lab-on-valve (SI-LOV) system was used to develop a new methodology for the determination of iron in wine samples exploiting the bead injection (BI) concept for solid phase extraction and spectrophotometric measurement. Nitrilotriacetic Acid (NTA) Superflow resin was used to build the bead column of the flow through sensor. The iron (III) ions were retained by the bead column and react with SCN- producing an intense red colour. The change in absorbance was monitored spectrophotometrically on the optosensor at 480 nm. It was possible to achieve a linear range of 0.09-5.0 mg L-1 of iron, with low sample and reagent consumption; 500 μL of sample, 15 μmol of SCN-, and 9 μmol of H2O2, per assay. The proposed method was successfully applied to the determination of iron in wine, with no previous treatment other than dilution, and to other food samples.

KW - Bead injection

KW - Iron

KW - Sequential injection lab-on-valve

KW - Solid phase spectrometry

KW - Wine

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SN - 0039-9140

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JF - Talanta

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Y2 - 25 April 2010 through 30 April 2010

ER -

Exploiting the bead injection LOV approach to carry out spectrophotometric assays in wine: application to the determination of iron

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