Exploiting the bead injection LOV approach to carry out spectrophotometric assays in wine: application to the determination of iron

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Abstract

A sequential injection lab-on-valve (SI-LOV) system was used to develop a new methodology for the determination of iron in wine samples exploiting the bead injection (BI) concept for solid phase extraction and spectrophotometric measurement. Nitrilotriacetic Acid (NTA) Superflow resin was used to build the bead column of the flow through sensor. The iron (III) ions were retained by the bead column and react with SCN- producing an intense red colour. The change in absorbance was monitored spectrophotometrically on the optosensor at 480 nm. It was possible to achieve a linear range of 0.09-5.0 mg L-1 of iron, with low sample and reagent consumption; 500 μL of sample, 15 μmol of SCN-, and 9 μmol of H2O2, per assay. The proposed method was successfully applied to the determination of iron in wine, with no previous treatment other than dilution, and to other food samples.
Original languageEnglish
Pages (from-to)1298-1303
Number of pages6
JournalTalanta
Volume84
Issue number5
DOIs
Publication statusPublished - 15 Jun 2011
Event16th International Conference on Flow Injection Analysis - , Thailand
Duration: 25 Apr 201030 Apr 2010
Conference number: 16

Keywords

  • Bead injection
  • Iron
  • Sequential injection lab-on-valve
  • Solid phase spectrometry
  • Wine

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