@article{225debb8c9994b25ad81b552f6fd652c,
title = "Exploration of bacterial Bottlenecks and Streptococcus pneumoniae pathogenesis by CRISPRi-Seq",
abstract = "Liu et al. developed CRISPRi-seq to enable in vivo genome-wide fitness testing of Streptococcus pneumoniae in one sequencing step. CRISPRi-seq revealed a bottleneck during murine pneumococcal infection not observed upon influenza virus co-infection, enabling identification of essential genes. By testing all genes, including essential genes, CRISPRi-seq has broad utility.",
keywords = "Bacterial pathogenesis, Bottleneck, CRISPRi-seq, Influenza A virus superinfection, Pneumonia, Streptococcus pneumoniae",
author = "Xue Liu and Kimmey, \{Jacqueline M.\} and Laura Matarazzo and \{de Bakker\}, Vincent and \{Van Maele\}, Laurye and Sirard, \{Jean Claude\} and Victor Nizet and Veening, \{Jan Willem\}",
note = "Funding Information: We appreciate all members of the Veening lab for stimulating discussions and thank Lance Keller for feedback on the manuscript. We thank Indiana Castro and Charlotte Costa for technical help on the IAV co-infection model. This work was supported by the Swiss National Science Foundation (SNSF) (project grant 31003A\_172861 to J.W.V.), SNSF JPIAMR grant (40AR40\_185533 to J.W.V.), and SNSF NCCR “AntiResist” (51NF40\_180541 to J.W.V.). Work in the Nizet lab is supported by the NIH grant AI145325. J.M.K. was supported by the University of California President's Postdoctoral Fellowship Program (UC PPFP). J.C.S. received funding from Inserm, University of Lille, Institut Pasteur de Lille and the European Union's Horizon 2020 research and innovation program under grant agreement no 847786. Conceptualization, X.L. J.M.K. and J.W.V.; Methodology, X.L. J.M.K. V.D.B. L.M. and L.V.M.; Formal Analysis, X.L. V.D.B. J.W.V. and J.M.K.; Writing – Original Draft, X.L. and J.W.V.; Writing – Review \& Editing, X.L. J.M.K. V.D.B. V.N. J.C.S. and J.W.V.; Funding Acquisition, J.W.V. V.N. J.M.K, and J.C.S.; Supervision, J.W.V. V.N. and J.C.S. The authors declare no conflicting interests. Funding Information: We appreciate all members of the Veening lab for stimulating discussions and thank Lance Keller for feedback on the manuscript. We thank Indiana Castro and Charlotte Costa for technical help on the IAV co-infection model. This work was supported by the Swiss National Science Foundation (SNSF) (project grant 31003A\_172861 to J.W.V.), SNSF JPIAMR grant ( 40AR40\_185533 to J.W.V.), and SNSF NCCR “AntiResist” ( 51NF40\_180541 to J.W.V.). Work in the Nizet lab is supported by the NIH grant AI145325 . J.M.K. was supported by the University of California President{\textquoteright}s Postdoctoral Fellowship Program (UC PPFP). J.C.S. received funding from Inserm , University of Lille , Institut Pasteur de Lille and the European Union{\textquoteright}s Horizon 2020 research and innovation program under grant agreement no 847786. Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = jan,
day = "13",
doi = "10.1016/j.chom.2020.10.001",
language = "English",
volume = "29",
pages = "107--120.e6",
journal = "Cell Host and Microbe",
issn = "1931-3128",
publisher = "Cell Press",
number = "1",
}
