TY - JOUR
T1 - Exploring antibiotic resistance in environmental integron-cassettes through intI-attC amplicons deep sequencing
AU - Dias, Marcela França
AU - Castro, Giovanni Marques de
AU - Paiva, Magna Cristina de
AU - Reis, Mariana de Paula
AU - Facchin, Susanne
AU - Carmo, Anderson Oliveira do
AU - Alves, Marta Salgueiro
AU - Suhadolnik, Maria Luíza
AU - de Moraes Motta, Amanda
AU - Henriques, Isabel
AU - Kalapothakis, Evanguedes
AU - Lobo, Francisco Pereira
AU - Nascimento, Andréa Maria Amaral
N1 - Funding Information:
This research was funded in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001, and by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant number 306774/2016-0), the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG, grant number APQ-01673-16), and the Pró-Reitoria de Pesquisa da UFMG (PRPq). Thanks are due for the financial support to CESAM (UID/AMB/50017/2019), to FCT/MEC through national funds, and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020.
Publisher Copyright:
© 2020, Sociedade Brasileira de Microbiologia.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2021/3
Y1 - 2021/3
N2 - Introduction: Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process. Material and methods: In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through intI-attC and 16S rRNA amplicons deep sequencing. Results and discussion: ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative novel ARG-cassettes homologs to fosB3 and novA were detected. Regarding 16SrRNA gene analysis, taxonomic and functional profiles unveiled Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as dominant phyla. Betaproteobacteria, Alphaproteobacteria, and Actinobacteria classes were the main contributors for KEGG orthologs associated with resistance. Conclusions: Overall, these results provide new information about environmental integrons as a source of resistance determinants outside clinical settings and the bacterial community in the Velhas River.
AB - Introduction: Freshwater ecosystems provide propitious conditions for the acquisition and spread of antibiotic resistance genes (ARGs), and integrons play an important role in this process. Material and methods: In the present study, the diversity of putative environmental integron-cassettes, as well as their potential bacterial hosts in the Velhas River (Brazil), was explored through intI-attC and 16S rRNA amplicons deep sequencing. Results and discussion: ORFs related to different biological processes were observed, from DNA integration to oxidation-reduction. ARGs-cassettes were mainly associated with class 1 mobile integrons carried by pathogenic Gammaproteobacteria, and possibly sedentary chromosomal integrons hosted by Proteobacteria and Actinobacteria. Two putative novel ARG-cassettes homologs to fosB3 and novA were detected. Regarding 16SrRNA gene analysis, taxonomic and functional profiles unveiled Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria as dominant phyla. Betaproteobacteria, Alphaproteobacteria, and Actinobacteria classes were the main contributors for KEGG orthologs associated with resistance. Conclusions: Overall, these results provide new information about environmental integrons as a source of resistance determinants outside clinical settings and the bacterial community in the Velhas River.
KW - 16S rRNA gene
KW - Antibiotic resistance
KW - Environmental integron-cassettes
KW - Urban river microbiome
UR - http://www.scopus.com/inward/record.url?scp=85096744377&partnerID=8YFLogxK
U2 - 10.1007/s42770-020-00409-8
DO - 10.1007/s42770-020-00409-8
M3 - Article
C2 - 33247398
AN - SCOPUS:85096744377
SN - 1517-8382
VL - 52
SP - 363
EP - 372
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 1
ER -