TY - JOUR
T1 - Expression of genes encoding extracellular matrix macromolecules and metalloproteinases in avian tibial dyschondroplasia
AU - Velada, I.
AU - Capela-Silva, F.
AU - Reis, F.
AU - Pires, E.
AU - Egas, C.
AU - Rodrigues-Santos, P.
AU - Barros, M. T.
PY - 2011/8
Y1 - 2011/8
N2 - Avian tibial dyschondroplasia (TD) is a skeletal disease characterized by disruption of endochondral bone formation. The aim of this study was to determine the expression of extracellular matrix (ECM) macromolecules and ECM-degrading enzymes [matrix metalloproteinases (MMPs)] in the growth plates of normal and TD-affected 3-week-old broiler chicks (Cobb strain). Protein levels were analyzed by immunoblotting and gelatin zymography and gene expression by polymerase chain reaction. Expression of genes encoding the ECM macromolecules (collagen types II, IX, X and XI; and aggrecan) was not altered in dyschondroplasia; however, there was down-regulation of genes encoding MMP-9, MMP-13, MMP-10 and MMP-11 in addition to reduced amounts of MMP-2 and MMP-13 proteins. In contrast, there was up-regulation of genes encoding MMP-7 and the vascular endothelial growth factor. These findings suggest that the accumulation of cartilage associated with the disease may be the result of decreased proteolysis due to the down-regulation of MMPs and not to an increased production of ECM macromolecules.
AB - Avian tibial dyschondroplasia (TD) is a skeletal disease characterized by disruption of endochondral bone formation. The aim of this study was to determine the expression of extracellular matrix (ECM) macromolecules and ECM-degrading enzymes [matrix metalloproteinases (MMPs)] in the growth plates of normal and TD-affected 3-week-old broiler chicks (Cobb strain). Protein levels were analyzed by immunoblotting and gelatin zymography and gene expression by polymerase chain reaction. Expression of genes encoding the ECM macromolecules (collagen types II, IX, X and XI; and aggrecan) was not altered in dyschondroplasia; however, there was down-regulation of genes encoding MMP-9, MMP-13, MMP-10 and MMP-11 in addition to reduced amounts of MMP-2 and MMP-13 proteins. In contrast, there was up-regulation of genes encoding MMP-7 and the vascular endothelial growth factor. These findings suggest that the accumulation of cartilage associated with the disease may be the result of decreased proteolysis due to the down-regulation of MMPs and not to an increased production of ECM macromolecules.
KW - Extracellular matrix
KW - Gene expression
KW - Growth plate
KW - Matrix metalloproteinases
KW - Tibial dyschondroplasia
UR - http://www.scopus.com/inward/record.url?scp=79960233347&partnerID=8YFLogxK
U2 - 10.1016/j.jcpa.2010.12.008
DO - 10.1016/j.jcpa.2010.12.008
M3 - Article
C2 - 21310429
AN - SCOPUS:79960233347
SN - 0021-9975
VL - 145
SP - 174
EP - 186
JO - Journal of Comparative Pathology
JF - Journal of Comparative Pathology
IS - 2-3
ER -