Extending the reservoir of bla IMP-5: the emerging pathogen Acinetobacter bereziniae

Filipa Grosso; Liliana Silva; Clara Sousa; Helena Ramos; Sandra Quinteira; Luísa Peixe

doi:10.2217/fmb.15.88

Extending the reservoir of bla _IMP-5: the emerging pathogen Acinetobacter bereziniae

Filipa Grosso, Liliana Silva, Clara Sousa, Helena Ramos, Sandra Quinteira, Luísa Peixe^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Aim: Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates. Materials & methods: Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations. Results: Isolates were not clonally related but both produced the 'exclusively Portuguese' IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located. Conclusion: Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens.

Original language	English
Pages (from-to)	1609-1613
Number of pages	5
Journal	Future Microbiology
Volume	10
Issue number	10
DOIs	https://doi.org/10.2217/fmb.15.88
Publication status	Published - Oct 2015
Externally published	Yes

Keywords

Acinetobacter bereziniae
Carbapenem resistance
IMP-5
Integron
Metallo-β-lactamase
Oxacillinase

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title = "Extending the reservoir of bla IMP-5: the emerging pathogen Acinetobacter bereziniae",

abstract = "Aim: Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates. Materials & methods: Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations. Results: Isolates were not clonally related but both produced the 'exclusively Portuguese' IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located. Conclusion: Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens.",

keywords = "Acinetobacter bereziniae, Carbapenem resistance, IMP-5, Integron, Metallo-β-lactamase, Oxacillinase",

author = "Filipa Grosso and Liliana Silva and Clara Sousa and Helena Ramos and Sandra Quinteira and Lu{\'i}sa Peixe",

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year = "2015",

month = oct,

doi = "10.2217/fmb.15.88",

language = "English",

volume = "10",

pages = "1609--1613",

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T1 - Extending the reservoir of bla IMP-5

T2 - the emerging pathogen Acinetobacter bereziniae

AU - Grosso, Filipa

AU - Silva, Liliana

AU - Sousa, Clara

AU - Ramos, Helena

AU - Quinteira, Sandra

AU - Peixe, Luísa

PY - 2015/10

Y1 - 2015/10

N2 - Aim: Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates. Materials & methods: Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations. Results: Isolates were not clonally related but both produced the 'exclusively Portuguese' IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located. Conclusion: Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens.

AB - Aim: Acinetobacter bereziniae clinical relevance is starting to be recognized; however, very few descriptions of its carbapenem resistance currently exist. Here we characterize two carbapenem-resistant A. bereziniae isolates. Materials & methods: Isolates were obtained from environmental and clinical samples. Carbapenemases were searched by phenotypic, biochemical and PCR assays. Clonality was studied by ApaI-PFGE and genetic location for carbapenemase genes were assessed by I-CeuI and S1 hybridizations. Results: Isolates were not clonally related but both produced the 'exclusively Portuguese' IMP-5, with the clinical isolate also producing an OXA-58. The carbapenemase genes were plasmid located. Conclusion: Our results emphasize the role of non-baumannii Acinetobacter species as important reservoirs of clinically relevant resistance genes that could also contribute to their emergence as nosocomial pathogens.

KW - Acinetobacter bereziniae

KW - Carbapenem resistance

KW - IMP-5

KW - Integron

KW - Metallo-β-lactamase

KW - Oxacillinase

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JO - Future Microbiology

JF - Future Microbiology

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ER -

Extending the reservoir of bla _IMP-5: the emerging pathogen Acinetobacter bereziniae

Abstract

Keywords

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