TY - JOUR
T1 - Field and classroom initiatives for portable sequence-based monitoring of dengue virus in Brazil
AU - Latin American Genomic Surveillance Arboviral Network
AU - Adelino, Talita Émile Ribeiro
AU - Giovanetti, Marta
AU - Fonseca, Vagner
AU - Xavier, Joilson
AU - de Abreu, Álvaro Salgado
AU - do Nascimento, Valdinete Alves
AU - Demarchi, Luiz Henrique Ferraz
AU - Oliveira, Marluce Aparecida Assunção
AU - da Silva, Vinícius Lemes
AU - de Mello, Arabela Leal e.Silva
AU - Cunha, Gabriel Muricy
AU - Santos, Roselene Hans
AU - de Oliveira, Elaine Cristina
AU - Júnior, Jorge Antônio Chamon
AU - de Melo Iani, Felipe Campos
AU - de Filippis, Ana Maria Bispo
AU - de Abreu, André Luiz
AU - de Jesus, Ronaldo
AU - de Albuquerque, Carlos Frederico Campelo
AU - Rico, Jairo Mendez
AU - do Carmo Said, Rodrigo Fabiano
AU - Silva, Joscélio Aguiar
AU - de Moura, Noely Fabiana Oliveira
AU - Leite, Priscila
AU - Frutuoso, Lívia Carla Vinhal
AU - Haddad, Simone Kashima
AU - Martínez, Alexander
AU - Barreto, Fernanda Khouri
AU - Vazquez, Cynthia Carolina
AU - da Cunha, Rivaldo Venâncio
AU - Araújo, Emerson Luiz Lima
AU - de Oliveira Tosta, Stephane Fraga
AU - de Araújo Fabri, Allison
AU - Chalhoub, Flávia Löwen Levy
AU - da Silva Lemos, Poliana
AU - de Bruycker-Nogueira, Fernanda
AU - de Castro Lichs, Gislene Garcia
AU - Zardin, Marina Castilhos Souza Umaki
AU - Segovia, Fátima María Cardozo
AU - Gonçalves, Crhistinne Cavalheiro Maymone
AU - Grillo, Zoraida Del Carmen Fernandez
AU - Slavov, Svetoslav Nanev
AU - Pereira, Luiz Augusto
AU - Mendonça, Ana Flávia
AU - Pereira, Felicidade Mota
AU - de Magalhães, Jurandy Júnior Ferraz
AU - dos Santos Júnior, Agenor de Castro Moreira
AU - de Lima, Maricélia Maia
AU - Nogueira, Rita Maria Ribeiro
AU - Lourenço, José
N1 - Funding Information:
mented an arbovirus surveillance project that took place across the Midwest of Brazil using a mobile genomics laboratory (Supplementary Fig. 9). This Brazilian-driven initiative, known as the ZiBRA-2 project, was supported by the BrMoH (https://www.zibra2project.org).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12/1
Y1 - 2021/12/1
N2 - Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015–2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses.
AB - Brazil experienced a large dengue virus (DENV) epidemic in 2019, highlighting a continuous struggle with effective control and public health preparedness. Using Oxford Nanopore sequencing, we led field and classroom initiatives for the monitoring of DENV in Brazil, generating 227 novel genome sequences of DENV1-2 from 85 municipalities (2015–2019). This equated to an over 50% increase in the number of DENV genomes from Brazil available in public databases. Using both phylogenetic and epidemiological models we retrospectively reconstructed the recent transmission history of DENV1-2. Phylogenetic analysis revealed complex patterns of transmission, with both lineage co-circulation and replacement. We identified two lineages within the DENV2 BR-4 clade, for which we estimated the effective reproduction number and pattern of seasonality. Overall, the surveillance outputs and training initiative described here serve as a proof-of-concept for the utility of real-time portable sequencing for research and local capacity building in the genomic surveillance of emerging viruses.
UR - http://www.scopus.com/inward/record.url?scp=85104412509&partnerID=8YFLogxK
U2 - 10.1038/s41467-021-22607-0
DO - 10.1038/s41467-021-22607-0
M3 - Article
C2 - 33863880
AN - SCOPUS:85104412509
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 2296
ER -
