Flow analysis method for hydroxyproline determination to assess collagen content in fish skin

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Abstract

Collagen is a protein with several applications, with weak antigenicity, low toxicity, and high nutritional value. Usually, it is extracted from bovine skin, but a project was designed proposing an alternative to extract collagen from fish skin. This alternative was tested by assessing the collagen content in fish skin based on the determination of hydroxyproline (HYP), one of the most abundant amino acid present in collagen (Sotelo et al., 2016). Therefore, the determination of HYP requires the hydrolysis of the fish skin, to break collagen in its amino acids, and the HYP value quantified relates to the collagen content. This was previously assessed to be 38 μg of HYP per mg of pure collagen. In this context, the aim of the described work was to develop an automatic method based on flow injection analysis approach to determine HYP. The determination was based on the redox reaction with permanganate (Farokhi et al., 2002) and the consequent decrease of colour intensity registered. The best conditions for the determination were studied, namely, reagent concentration, sample volume, flow rate and reaction time. The developed method enables the determination of the HYP in a faster, simpler and accurate way, with less toxic solutions.
Original languageEnglish
Pages30-30
Number of pages1
Publication statusPublished - May 2023
Event22nd International Conference on Flow Injection Analysis - Marseille, France, Marseille, France
Duration: 28 May 20232 Jun 2023

Conference

Conference22nd International Conference on Flow Injection Analysis
Abbreviated titleICFIA 2023
Country/TerritoryFrance
CityMarseille
Period28/05/232/06/23

Keywords

  • Flow injection analysis
  • Hydroxyproline
  • Permanganate
  • Blue shark

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