Flow-based system for the determination of titratable acidity in wines

Susana S. M. P. Vidigal*, Ana T. C. Ramos, António O. S. S. Rangel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


A flow injection analysis system with a spectrophotometric detection was arranged in order to develop a simple and fast methodology for the quantification of titratable acidity in different types of wines. In the proposed system, a precise volume of sample is injected in the carrier stream, composed by a mixture of OH− solution with the pH indicator, bromothymol blue (BTB). Consequently, a change on the colour of the carrier solution is achieved and quantified by spectrophotometry. Therefore, it was possible to establish a linear range up to 0.16 g/L expressed as tartaric acid, with a limit of detection and quantification of 0.01 and 0.05 g/L, respectively. The proposed work has a low sample and reagents consumption, only 96 μg of BTB, 336 mmol de OH− and 50 μL of sample were needed per assay. At the same time, a high determination rate was achieved, 75 determinations per hour. The developed methodology was applied on the quantification of total acidity in red and white table wine and port wine samples.
Original languageEnglish
Pages (from-to)2241-2245
Number of pages5
JournalFood Analytical Methods
Issue number8
Publication statusPublished - 1 Aug 2016


  • Bromothymol blue
  • Flow-injection system
  • Spectrophotometry
  • Table and port wine
  • Titratable acidity


Dive into the research topics of 'Flow-based system for the determination of titratable acidity in wines'. Together they form a unique fingerprint.

Cite this