Fluconazole and voriconazole resistance in Candida parapsilosis is conferred by gain-of-function mutations in MRR1 transcription factor gene

Joana Branco; Ana P. Silva; Raquel M. Silva; Ana Silva-Dias; Cidália Pina-Vaz; Geraldine Butler; Acácio G. Rodrigues; Isabel M. Miranda

doi:10.1128/AAC.00842-15

Fluconazole and voriconazole resistance in Candida parapsilosis is conferred by gain-of-function mutations in MRR1 transcription factor gene

Joana Branco, Ana P. Silva, Raquel M. Silva, Ana Silva-Dias, Cidália Pina-Vaz, Geraldine Butler, Acácio G. Rodrigues, Isabel M. Miranda^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Candida parapsilosis is the second most prevalent fungal agent causing bloodstream infections. Nevertheless, there is little information about the molecular mechanisms underlying azole resistance in this species. Mutations (G1747A, A2619C, and A3191C) in the MRR1 transcription factor gene were identified in fluconazole-and voriconazole-resistant strains. Independent expression of MRR1 genes harboring these mutations showed that G1747A (G583R) and A2619C (K873N) are gain-of-function mutations responsible for azole resistance, the first described in C. parapsilosis.

Original language	English
Pages (from-to)	6629-6633
Number of pages	5
Journal	Antimicrobial Agents and Chemotherapy
Volume	59
Issue number	10
DOIs	https://doi.org/10.1128/AAC.00842-15
Publication status	Published - 1 Oct 2015
Externally published	Yes

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title = "Fluconazole and voriconazole resistance in Candida parapsilosis is conferred by gain-of-function mutations in MRR1 transcription factor gene",

abstract = "Candida parapsilosis is the second most prevalent fungal agent causing bloodstream infections. Nevertheless, there is little information about the molecular mechanisms underlying azole resistance in this species. Mutations (G1747A, A2619C, and A3191C) in the MRR1 transcription factor gene were identified in fluconazole-and voriconazole-resistant strains. Independent expression of MRR1 genes harboring these mutations showed that G1747A (G583R) and A2619C (K873N) are gain-of-function mutations responsible for azole resistance, the first described in C. parapsilosis.",

author = "Joana Branco and Silva, {Ana P.} and Silva, {Raquel M.} and Ana Silva-Dias and Cid{\'a}lia Pina-Vaz and Geraldine Butler and Rodrigues, {Ac{\'a}cio G.} and Miranda, {Isabel M.}",

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T1 - Fluconazole and voriconazole resistance in Candida parapsilosis is conferred by gain-of-function mutations in MRR1 transcription factor gene

AU - Branco, Joana

AU - Silva, Ana P.

AU - Silva, Raquel M.

AU - Silva-Dias, Ana

AU - Pina-Vaz, Cidália

AU - Butler, Geraldine

AU - Rodrigues, Acácio G.

AU - Miranda, Isabel M.

PY - 2015/10/1

Y1 - 2015/10/1

N2 - Candida parapsilosis is the second most prevalent fungal agent causing bloodstream infections. Nevertheless, there is little information about the molecular mechanisms underlying azole resistance in this species. Mutations (G1747A, A2619C, and A3191C) in the MRR1 transcription factor gene were identified in fluconazole-and voriconazole-resistant strains. Independent expression of MRR1 genes harboring these mutations showed that G1747A (G583R) and A2619C (K873N) are gain-of-function mutations responsible for azole resistance, the first described in C. parapsilosis.

AB - Candida parapsilosis is the second most prevalent fungal agent causing bloodstream infections. Nevertheless, there is little information about the molecular mechanisms underlying azole resistance in this species. Mutations (G1747A, A2619C, and A3191C) in the MRR1 transcription factor gene were identified in fluconazole-and voriconazole-resistant strains. Independent expression of MRR1 genes harboring these mutations showed that G1747A (G583R) and A2619C (K873N) are gain-of-function mutations responsible for azole resistance, the first described in C. parapsilosis.

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AN - SCOPUS:84942860792

SN - 0066-4804

VL - 59

SP - 6629

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

ER -

Fluconazole and voriconazole resistance in Candida parapsilosis is conferred by gain-of-function mutations in MRR1 transcription factor gene

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