Projects per year
Bacille Calmette-Guérin (BCG), the only currently licenced tuberculosis vaccine, may exert beneficial non-specific effects (NSE) in reducing infant mortality. We conducted a randomised controlled clinical study in healthy UK adults to evaluate potential NSE using functional in-vitro growth inhibition assays (GIAs) as a surrogate of protection from four bacteria implicated in infant mortality. Volunteers were randomised to receive BCG intradermally (n = 27) or to be unvaccinated (n = 8) and were followed up for 84 days; laboratory staff were blinded until completion of the final visit. Using GIAs based on peripheral blood mononuclear cells, we observed a significant reduction in the growth of the Gram-negative bacteria Escherichia coli and Klebsiella pneumonia following BCG vaccination, but no effect for the Gram-positive bacteria Staphylococcus aureus and Streptococcus agalactiae. There was a modest association between S. aureus nasal carriage and growth of S. aureus in the GIA. Our findings support a causal link between BCG vaccination and improved ability to control growth of heterologous bacteria. Unbiased assays such as GIAs are potentially useful tools for the assessment of non-specific as well as specific effects of TB vaccines. This study was funded by the Bill and Melinda Gates Foundation and registered with ClinicalTrials.gov (NCT02380508, 05/03/2015; completed).
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Barros, M., Rosa, N., Correia, M. J., Caldas, A. C., Amado, J. C., Figueiredo, A. S., Esteves, A. C., Mineiro, A., Abreu, A. M., Duarte, A. S., Almeida, S. F., Correia, A., Moura, A., Almeida, A., Araújo, B., Moura-Netto, C., Ferrito, C., Pais-Vieira, C., Festas, C., Marques-Vieira, C., Catré, D., Nunes, E., Jesus, É., Ribeiro, F., Rosário, F., Fernandes, G., Rato, J. R., Salgado, J. R., Neves-Amado, J., Amendoeira, J., Sá, L., Capelas, M. L., Vieira, M. M., Nunes, M. V. S., Cardoso, M., Veiga, N. J., Fonseca, P., Correia, P. N., Couto, P., Pontífice-Sousa, P., Ravasco, P., Carvalho, P. V. D., Alves, P., Melo, P., Silva, R., Canaipa, R., Noites, R., Rio, R., Almeida, S., Deodato, S., Caldeira, S., Silva, S., Borges, T., Silva, V., Charepe, Z., Rodrigues-Pires, F., Veludo, F., Carmo, H., Romeiro, J., Melo, M., Braga, M., Amaral, T., Moreira, M. A., Guerra, N., Santos, P., Paço, S., Lynce, S., Miguel, S., Costa, T., Silva-Neves, V., Silva, A., Carvalho, A. R., Almeida, B., Figueiredo, C., Esteves, E., Araújo, F. M., Garcia, J. G., Santos, L., Santos, N. M. D., Lopes, P., Bornes, R., Silva, R., Costa, S., Silva, S. M., Marques, T., Almeida, A., Santos, M., Santos, P., Miguel, S., Mendes, A. K. D. S., Gomes, A. T. P. D. C., Henriques, J. M. P., Costa, H. A. F. P. & Ribeiro, P. A. D. O. C.
1/01/20 → 31/12/24