TY - JOUR
T1 - Gas chromatography - Optical fiber detector for assessment of fatty acids in urban soils
AU - Silva, Lurdes
AU - Cachada, Anabela
AU - Pereira, Ruth
AU - Freitas, Ana Cristina
AU - Rocha-Santos, Teresa A. P.
AU - Panteleitchouk, Teresa S L.
AU - Pereira, Maria E.
AU - Duarte, Armando Costa
PY - 2011/7/15
Y1 - 2011/7/15
N2 - Fatty acids have been used as biomarkers of the microbial community composition of soils and they are usually separated and quantified by gas-chromatography coupled to a flame ionization detector (GC-FID). The aim of this study was to develop, validate and apply a methodology based on gas chromatography coupled to optical fiber detection (GC-OF) for screening five fatty acids used as indicators of fungal and bacterial communities in urban soils. The performance of the GC-OF methodology (optical fiber detector at 1550 nm) was evaluated by comparison with the GC-FID methodology and it was found that they were comparable in terms of linear range, detection limit and analytical errors. Besides these similar analytical characteristics, the GC-OF is much cheaper than the GC-FID methodology. Different concentrations were determined for each fatty acid indicator which in turn varied significantly between the soil samples analyzed from Lisbon ornamental gardens. Additionally, the GC-OF showed a great potential as alternative for determination of eleven or more fatty acids in urban soils.
AB - Fatty acids have been used as biomarkers of the microbial community composition of soils and they are usually separated and quantified by gas-chromatography coupled to a flame ionization detector (GC-FID). The aim of this study was to develop, validate and apply a methodology based on gas chromatography coupled to optical fiber detection (GC-OF) for screening five fatty acids used as indicators of fungal and bacterial communities in urban soils. The performance of the GC-OF methodology (optical fiber detector at 1550 nm) was evaluated by comparison with the GC-FID methodology and it was found that they were comparable in terms of linear range, detection limit and analytical errors. Besides these similar analytical characteristics, the GC-OF is much cheaper than the GC-FID methodology. Different concentrations were determined for each fatty acid indicator which in turn varied significantly between the soil samples analyzed from Lisbon ornamental gardens. Additionally, the GC-OF showed a great potential as alternative for determination of eleven or more fatty acids in urban soils.
KW - Fatty acids
KW - GC-FID
KW - GC-OF
KW - Urban soil
UR - http://www.scopus.com/inward/record.url?scp=79958168714&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2011.03.044
DO - 10.1016/j.talanta.2011.03.044
M3 - Article
AN - SCOPUS:79958168714
SN - 0039-9140
VL - 85
SP - 222
EP - 229
JO - Talanta
JF - Talanta
IS - 1
ER -
