TY - JOUR
T1 - Gene expression and physiological responses associated to stomatal functioning in Rosa × hybrida grown at high relative air humidity
AU - Carvalho, Dália R. A.
AU - Vasconcelos, Marta W.
AU - Lee, Sangseok
AU - Koning-Boucoiran, Carole F. S.
AU - Vreugdenhil, Dick
AU - Krens, Frans A.
AU - Heuvelink, Ep
AU - Carvalho, Susana M. P.
N1 - Funding Information:
This work received financial support from FCT-Portuguese Foundation for Science and Technology (Projects UID/Multi/50016/2013; UID/AGR/04033/2013 and PhD scholarship SFRH/BD/72924/2010 to D.R.A. Carvalho) and from European Investment Funds FEDER/COMPETE/POCI– Operational Competitiveness and Internationalization Program (Project POCI-01-0145-FEDER-006958). The authors acknowledge Dümmen Orange (The Netherlands) for generating the K5 population and making it available. Thanks also to Giulia Lucaroni for helping conducting the stomatal images and to Julian Verdonk for helping with the program LinRegPCR.
Publisher Copyright:
© 2016 Elsevier Ireland Ltd
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2016/12/1
Y1 - 2016/12/1
N2 - High relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%) or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.
AB - High relative air humidity (RH ≥ 85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62 ± 3%) or high (89 ± 4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.
KW - Abscisic acid
KW - Stomatal anatomy
KW - Tetraploid roses
KW - Vapor pressure deficit
KW - Water loss
UR - http://www.scopus.com/inward/record.url?scp=84991772108&partnerID=8YFLogxK
U2 - 10.1016/j.plantsci.2016.09.018
DO - 10.1016/j.plantsci.2016.09.018
M3 - Article
C2 - 27968984
AN - SCOPUS:84991772108
SN - 0168-9452
VL - 253
SP - 154
EP - 163
JO - Plant Science
JF - Plant Science
ER -