Genome-wide analysis of nuclear mRNA export pathways in Drosophila

Andrea Herold, Luis Teixeira, Elisa Izaurralde*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

132 Citations (Scopus)

Abstract

NXF1, p15 and UAP56 are essential nuclear mRNA export factors. The fraction of mRNAs exported by these proteins or via alternative pathways is unknown. We have analyzed the relative abundance of nearly half of the Drosophila transcriptome in the cytoplasm of cells treated with the CRM1 inhibitor leptomycin-B (LMB) or depleted of export factors by RNA interference. While the vast majority of mRNAs were unaffected by LMB, the levels of most mRNAs were significantly reduced in cells depleted of NXF1, p15 or UAP56. The striking similarities of the mRNA expression profiles in NXF1, p15 and UAP56 knockdowns show that these proteins act in the same pathway. The broad effect on mRNA levels observed in these cells indicates that the functioning of this pathway is required for export of most mRNAs. Nonetheless, a set of mRNAs whose export was unaffected by the depletions and some requiring NXF1:p15 but not UAP56 were identified. In addition, our analysis revealed a feedback loop by which a block to mRNA export triggers the upregulation of genes involved in this process.

Original languageEnglish
Pages (from-to)2472-2483
Number of pages12
JournalEMBO Journal
Volume22
Issue number10
DOIs
Publication statusPublished - 15 May 2003
Externally publishedYes

Keywords

  • CRM1
  • MRNA export
  • Nuclear export
  • NXF1
  • UAP56

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