TY - JOUR
T1 - Genomic and epidemiological surveillance of Zika virus in the Amazon region
AU - Giovanetti, Marta
AU - Faria, Nuno Rodrigues
AU - Lourenço, José
AU - Goes de Jesus, Jaqueline
AU - Xavier, Joilson
AU - Claro, Ingra Morales
AU - Kraemer, Moritz U.G.
AU - Fonseca, Vagner
AU - Dellicour, Simon
AU - Thézé, Julien
AU - da Silva Salles, Flavia
AU - Gräf, Tiago
AU - Silveira, Paola Paz
AU - do Nascimento, Valdinete Alves
AU - Costa de Souza, Victor
AU - de Melo Iani, Felipe Campos
AU - Castilho-Martins, Emerson Augusto
AU - Cruz, Laura Nogueira
AU - Wallau, Gabriel
AU - Fabri, Allison
AU - Levy, Flávia
AU - Quick, Joshua
AU - de Azevedo, Vasco
AU - Aguiar, Renato Santana
AU - de Oliveira, Tulio
AU - Bôtto de Menezes, Camila
AU - da Costa Castilho, Marcia
AU - Terra, Tirza Matos
AU - Souza da Silva, Marineide
AU - Bispo de Filippis, Ana Maria
AU - Luiz de Abreu, André
AU - Oliveira, Wanderson Kleber
AU - Croda, Julio
AU - Campelo de Albuquerque, Carlos F.
AU - Nunes, Marcio R.T.
AU - Sabino, Ester Cerdeira
AU - Loman, Nicholas
AU - Naveca, Felipe Gomes
AU - Pybus, Oliver G.
AU - Alcantara, Luiz Carlos
N1 - Funding Information:
We thank Oxford Nanopore Technologies for technical support and QIAGEN for support of the ZIBRA-2 (Zika in Brazil Real Time Analyses-Second Round) project with reagents and equipment. This work was supported by Decit/SCTIE/MoH and CNPq ( 440685/2016-8 and 440856/2016-7 ); by CAPES ( 88887.130716/2016-00 , 88881.130825/2016-00 , and 88887.130823/2016-00 ); by the European Union (EU) Horizon 2020 Programme through ZIKAlliance ( PRES-005-FEX-17-4-2-33 ); and by the Oxford Martin School . M.U.G.K. is supported by The Branco Weiss Fellowship - Society in Science, administered by ETH Zurich , and acknowledges funding from a Google Faculty Award and a Training Grant from the National Institute of Child Health and Human Development ( T32HD040128 ). S.D. is supported by Fonds National de la Recherche Scientifique (FNRS; Belgium). The activities of the A.M.B.F. laboratory were supported by the Faperj under the grant no. E-26/2002.930/2016 , by the International Development Research Centre (IDRC) Canada under the grant 108411-001 ) and the EU Horizon 2020 Programme through ZikaPlan and ZikAction (grants 734584 and 734857 ). M.G. is supported by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ).
Funding Information:
We thank Oxford Nanopore Technologies for technical support and QIAGEN for support of the ZIBRA-2 (Zika in Brazil Real Time Analyses-Second Round) project with reagents and equipment. This work was supported by Decit/SCTIE/MoH and CNPq (440685/2016-8 and 440856/2016-7); by CAPES (88887.130716/2016-00, 88881.130825/2016-00, and 88887.130823/2016-00); by the European Union (EU) Horizon 2020 Programme through ZIKAlliance (PRES-005-FEX-17-4-2-33); and by the Oxford Martin School. M.U.G.K. is supported by The Branco Weiss Fellowship - Society in Science, administered by ETH Zurich, and acknowledges funding from a Google Faculty Award and a Training Grant from the National Institute of Child Health and Human Development (T32HD040128). S.D. is supported by Fonds National de la Recherche Scientifique (FNRS; Belgium). The activities of the A.M.B.F. laboratory were supported by the Faperj under the grant no. E-26/2002.930/2016, by the International Development Research Centre (IDRC) Canada under the grant 108411-001) and the EU Horizon 2020 Programme through ZikaPlan and ZikAction (grants 734584 and 734857). M.G. is supported by Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ). Conception and Design, M.G. N.R.F. N.L. O.G.P. and L.C.A.; Investigations, M.G. N.R.F. F.G.N. J.G.J. J.X. I.M.C. F.S.S. P.P.S. V.A.N. V.C.S. F.C.M.I. G.W. E.A.C.-M. A.F. and F.L.; Data Curation, M.G. N.R.F. J.L. M.U.G.K. V.F. S.D. J.T. O.G.P. and L.C.J.A.; Formal Analysis, M.G. N.R.F. J.L. M.U.G.K. S.D. and L.P.; Writing – Original Draft Preparation, M.G. N.R.F. J.L. M.U.G.K. F.G.N. O.G.P. and L.C.J.A.; Revision, M.G. N.R.F. J.L. M.U.G.K. F.G.N. O.G.P. T.G. M.R.T.N. T.O. and L.C.J.A.; Resources, L.N.C. M.C.C. F.G.N. T.M.T. M.S.S. A.M.B.F. A.L.A. W.K.O. J.C. C.F.C.A. and L.C.J.A. The authors declare no competing interests.
Funding Information:
The project was supported by the Pan American World Health Organization (PAHO) and the Brazilian Ministry of Health (MoH) as part of the arboviral genomic surveillance efforts within the terms of Resolution 510/2016 of CONEP (Comissão Nacional de Ética em Pesquisa, Ministério da Saúde; National Ethical Committee for Research, Ministry of Health). The diagnostic of ZIKV infection at ILMD was approved by the Ethics Committee of the State University of Amazonas (CAAE: 56.745.116.6.0000.5016).
Publisher Copyright:
© 2020 The Authors
PY - 2020/2/18
Y1 - 2020/2/18
N2 - Zika virus (ZIKV) has caused an explosive epidemic linked to severe clinical outcomes in the Americas. As of June 2018, 4,929 ZIKV suspected infections and 46 congenital syndrome cases had been reported in Manaus, Amazonas, Brazil. Although Manaus is a key demographic hub in the Amazon region, little is known about the ZIKV epidemic there, in terms of both transmission and viral genetic diversity. Using portable virus genome sequencing, we generated 59 ZIKV genomes in Manaus. Phylogenetic analyses indicated multiple introductions of ZIKV from northeastern Brazil to Manaus. Spatial genomic analysis of virus movement among six areas in Manaus suggested that populous northern neighborhoods acted as sources of virus transmission to other neighborhoods. Our study revealed how the ZIKV epidemic was ignited and maintained within the largest urban metropolis in the Amazon. These results might contribute to improving the public health response to outbreaks in Brazil.
AB - Zika virus (ZIKV) has caused an explosive epidemic linked to severe clinical outcomes in the Americas. As of June 2018, 4,929 ZIKV suspected infections and 46 congenital syndrome cases had been reported in Manaus, Amazonas, Brazil. Although Manaus is a key demographic hub in the Amazon region, little is known about the ZIKV epidemic there, in terms of both transmission and viral genetic diversity. Using portable virus genome sequencing, we generated 59 ZIKV genomes in Manaus. Phylogenetic analyses indicated multiple introductions of ZIKV from northeastern Brazil to Manaus. Spatial genomic analysis of virus movement among six areas in Manaus suggested that populous northern neighborhoods acted as sources of virus transmission to other neighborhoods. Our study revealed how the ZIKV epidemic was ignited and maintained within the largest urban metropolis in the Amazon. These results might contribute to improving the public health response to outbreaks in Brazil.
UR - http://www.scopus.com/inward/record.url?scp=85079614093&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2020.01.085
DO - 10.1016/j.celrep.2020.01.085
M3 - Article
C2 - 32075736
AN - SCOPUS:85079614093
SN - 2211-1247
VL - 30
SP - 2275-2283.e7
JO - Cell reports
JF - Cell reports
IS - 7
ER -