TY - JOUR
T1 - Genomic and phylogenetic analysis of Salmonella enterica serovar Enteritidis strains linked to multiple outbreaks in Brazil
AU - Cao, Guojie
AU - Balkey, Maria
AU - Jin, Qing
AU - Brown, Eric
AU - Allard, Marc
AU - Melo, Adma Nadja Ferreira de
AU - Pedrosa, Geany Targino de Souza
AU - Oliveira, Tereza Cristina Rocha Moreira de
AU - Magnani, Marciane
AU - Macarisin, Dumitru
N1 - Publisher Copyright:
© 2022 The Author(s).
PY - 2023/1
Y1 - 2023/1
N2 - Salmonella enterica subsp. enterica serovar Enteritidis (SE) has become the prevalent serovar isolated from gastroenteritis cases in Brazil since the 1990s. To better understand the genomic diversity and phylogenetic relationship amongst SE epidemic isolates from Brazil, 30 SE isolates from a variety of implicated foods and case patients of outbreaks between 1999 and 2006 were selected for genome comparison analyses. SE genomes were also compared against publicly available Brazilian SE isolates from pre- and postepidemic period. MLST analysis revealed that all isolates belong to sequence type (ST) 11. A total of seven Salmonella pathogenicity islands (SPIs) (SPI-1, SPI-3–5, SPI-13, SPI14, and C63PI) were identified in the evaluated genomes and all studied SE genomes carried similar prophage profiling. Resistome analysis revealed the presence of resistance genes to aminoglycosides [aac(6’)laa, aph(3”)-lb, aph(6)-ld], as well as point mutations in gyrA. Phylogenetic analysis demonstrated that certain isolates have circulated in Brazil for years and been involved in distinct outbreaks.
AB - Salmonella enterica subsp. enterica serovar Enteritidis (SE) has become the prevalent serovar isolated from gastroenteritis cases in Brazil since the 1990s. To better understand the genomic diversity and phylogenetic relationship amongst SE epidemic isolates from Brazil, 30 SE isolates from a variety of implicated foods and case patients of outbreaks between 1999 and 2006 were selected for genome comparison analyses. SE genomes were also compared against publicly available Brazilian SE isolates from pre- and postepidemic period. MLST analysis revealed that all isolates belong to sequence type (ST) 11. A total of seven Salmonella pathogenicity islands (SPIs) (SPI-1, SPI-3–5, SPI-13, SPI14, and C63PI) were identified in the evaluated genomes and all studied SE genomes carried similar prophage profiling. Resistome analysis revealed the presence of resistance genes to aminoglycosides [aac(6’)laa, aph(3”)-lb, aph(6)-ld], as well as point mutations in gyrA. Phylogenetic analysis demonstrated that certain isolates have circulated in Brazil for years and been involved in distinct outbreaks.
KW - Salmonella
KW - Diversity
KW - Food safety
KW - Genomics
KW - Virulence
UR - http://www.scopus.com/inward/record.url?scp=85149182505&partnerID=8YFLogxK
U2 - 10.1093/lambio/ovac045
DO - 10.1093/lambio/ovac045
M3 - Article
C2 - 36688781
SN - 0266-8254
VL - 76
JO - Letters in Applied Microbiology
JF - Letters in Applied Microbiology
IS - 1
M1 - ovac045
ER -