TY - JOUR
T1 - Genomic investigation of antimicrobial resistance determinants and virulence factors in Salmonella enterica serovars isolated from contaminated food and human stool samples in Brazil
AU - Melo, Adma Nadja Ferreira de
AU - Monte, Daniel F. M.
AU - Pedrosa, Geany Targino de Souza
AU - Balkey, Maria
AU - Jin, Qing
AU - Brown, Eric
AU - Allard, Marc
AU - Oliveira, Tereza Cristina Rocha Moreira de
AU - Cao, Guojie
AU - Magnani, Marciane
AU - Macarisin, Dumitru
N1 - Funding Information:
Authors would like to thank the “ Conselho Nacional de Desenvolvimento Científico e Tecnológico ” (Grants # CNPq, Grants # 303437/2017-0 and # 405644/2018-3 ) and “ Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ” (CAPES-Brazil; Finance code 001 and UFPB/CAPES/PrInt 88881.311776/2018-01 ).
Funding Information:
Authors would like to thank the ?Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico? (Grants # CNPq, Grants #303437/2017-0 and #405644/2018-3) and ?Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior? (CAPES-Brazil; Finance code 001 and UFPB/CAPES/PrInt 88881.311776/2018-01).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/4/2
Y1 - 2021/4/2
N2 - This study investigated the antimicrobial resistance determinants, virulence factors and identified serovars in 37 Salmonella enterica strains isolated from human stool and contaminated foods linked to outbreaks that occurred in Brazil over 7 years using whole genome sequencing (WGS). Phylogenetic analysis of selected serovars (S. Typhimurium, S. Infantis, S. London, and S. Johannesburg) was performed. Ten distinct serovars were identified and, 51% of the tested strains (n = 19) showed disagreement with the previous conventional serotyping. The antimicrobial resistance (AMR) determinants or plasmids varied among the strains. Resistome analysis revealed the presence of resistance genes to aminoglycosides [aac (6′)-laa, aph (3″)-lb, aph (6)-ld, aadA1 and aadA2], sulfonamides (sul1), trimethoprin (dfrA8), fosfomycin (fosA7) and tetracyclines (tetA, tetB, tetC), as well as point mutations in parC (T57S) and gyrA (S83F). Plasmidome showed the presence of IncHI2, IncHI2A, IncFIB (S), IncFII (S), IncI1 and p0111 plasmids. Eight Salmonella pathogenicity islands and up to 102 stress and/or virulence genes were identified in the evaluated genomes. Virulence genes of K88 fimbrial adhesin were first reported in S. enterica (S. Pomona, S. Bredeney and S. Mbandaka strains). pilW gene was first identified in S. Pomona. Phylogenetic analysis showed that some serovars circulated in Brazil for decades, primarily within the poultry production chain. Findings highlighted the virulence and AMR determinants in strains that may lead to recurring food outbreaks.
AB - This study investigated the antimicrobial resistance determinants, virulence factors and identified serovars in 37 Salmonella enterica strains isolated from human stool and contaminated foods linked to outbreaks that occurred in Brazil over 7 years using whole genome sequencing (WGS). Phylogenetic analysis of selected serovars (S. Typhimurium, S. Infantis, S. London, and S. Johannesburg) was performed. Ten distinct serovars were identified and, 51% of the tested strains (n = 19) showed disagreement with the previous conventional serotyping. The antimicrobial resistance (AMR) determinants or plasmids varied among the strains. Resistome analysis revealed the presence of resistance genes to aminoglycosides [aac (6′)-laa, aph (3″)-lb, aph (6)-ld, aadA1 and aadA2], sulfonamides (sul1), trimethoprin (dfrA8), fosfomycin (fosA7) and tetracyclines (tetA, tetB, tetC), as well as point mutations in parC (T57S) and gyrA (S83F). Plasmidome showed the presence of IncHI2, IncHI2A, IncFIB (S), IncFII (S), IncI1 and p0111 plasmids. Eight Salmonella pathogenicity islands and up to 102 stress and/or virulence genes were identified in the evaluated genomes. Virulence genes of K88 fimbrial adhesin were first reported in S. enterica (S. Pomona, S. Bredeney and S. Mbandaka strains). pilW gene was first identified in S. Pomona. Phylogenetic analysis showed that some serovars circulated in Brazil for decades, primarily within the poultry production chain. Findings highlighted the virulence and AMR determinants in strains that may lead to recurring food outbreaks.
KW - Antibiotic resistance
KW - Poultry chain
KW - Salmonellosis
KW - Whole genome sequence
UR - http://www.scopus.com/inward/record.url?scp=85101421138&partnerID=8YFLogxK
U2 - 10.1016/j.ijfoodmicro.2021.109091
DO - 10.1016/j.ijfoodmicro.2021.109091
M3 - Article
C2 - 33639477
SN - 0168-1605
VL - 343
JO - International Journal of Food Microbiology
JF - International Journal of Food Microbiology
M1 - 109091
ER -